Mouse Connexin 43 ELISA Kit | MyBioSource MBS729401 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Connexin 43 ELISA Kit

catalog :

MBS729401

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS729401

products type :

ELISA Kit

products full name :

Mouse Connexin 43 ELISA Kit

products short name :

Connexin 43

other names :

Cx43 protein; Gap junction alpha-1 protein; gap junction alpha-1 protein; shf; sof; connexin-43; etID309742.9; short fin protein; gap junction protein, alpha 1; connexin 43; Connexin-43; Cx43; Short fin protein

products gene name :

CX43

other gene names :

cx43; gja1; gja1; cx43a1; zfCx43.3; cx43; shf; sof; Cx43

uniprot entry name :

CXA1_DANRE

reactivity :

Mouse

specificity :

This assay has high sensitivity and excellent specificity for detection of CX43. No significant cross-reactivity or interference between CX43 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CX43 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.

products categories :

Cell Biology

products description :

Intended Uses: This CX43 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CX43. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: CX43 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CX43. Standards or samples are then added to the microtiter plate wells and CX43 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CX43 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CX43 are added to each well to "sandwich" the CX43 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CX43 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CX43 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

29179440

ncbi acc num :

AAH49297.1

uniprot acc num :

O57474

ncbi mol weight :

43,455 Da

ncbi pathways :

Calcium Regulation In The Cardiac Cell Pathway (198967); EGFR1 Signaling Pathway (198963); Formation Of Annular Gap Junctions Pathway (365266); Gap Junction Pathway (119270); Gap Junction Pathway (483); Gap Junction Assembly Pathway (365260); Gap Junction Degradation Pathway (365265); Gap Junction Trafficking Pathway (365259); Gap Junction Trafficking And Regulation Pathway (365258); Membrane Trafficking Pathway (365251)

uniprot summary :

Function: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Ref.1Plays a role in regulation of fin bone size and growth. Ref.1. Subunit structure: A connexon is composed of a hexamer of connexins. Subcellular location: Cell membrane; Multi-pass membrane protein. Cell junction gap junction. Tissue specificity: Expressed in adult ovary, heart, lens and liver. In growing caudal fins, expressed in cells flanking the germinal region of newly growing segments and in osteoblasts at segment boundaries. Ref.1 Ref.2 Ref.5. Developmental stage: Expressed both maternally and zygotically. Zygotic expression is initiated before gastrulation and persists through to day 5 of development. Levels increase in late somitogenesis, and late embryonic and early larval stages. Embryonic expression is observed in many developing tissues and organs, including the notochord and brain, and heart and vasculature. Detected throughout the lens at 24 hours post-fertilization (hpf), but by 3-4 days development, restricted to the lateral epithelium and differentiating cells in the equatorial region. Ref.2 Ref.5. Miscellaneous: Morpholino knockdown of the protein results in heart folding defects, reduced hematopoiesis and small eyes. Sequence similarities: Belongs to the connexin family. Alpha-type (group II) subfamily.

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage