Human Stathmin 1 ELISA Kit | MyBioSource MBS729372 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Stathmin 1 ELISA Kit

catalog :

MBS729372

quantity :

48-Strip-Wells-(Comp

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS729372

products type :

ELISA Kit

products full name :

Human Stathmin 1 ELISA Kit

products short name :

Stathmin 1

other names :

stathmin 1, partial; Stathmin; stathmin; prosolin; metablastin; oncoprotein 18; phosphoprotein 19; phosphoprotein p19; stathmin 1/oncoprotein 18; transmembrane protein C1orf215; leukemia-associated phosphoprotein p18; stathmin 1; Leukemia-associated phosphoprotein p18; Metablastin; Oncoprotein 18; Op18; Phosphoprotein p19; pp19; Prosolin; Protein Pr22; pp17

products gene name :

STMN1

other gene names :

STMN1; STMN1; Lag; SMN; OP18; PP17; PP19; PR22; LAP18; C1orf215; C1orf215; LAP18; OP18; Op18; pp19

uniprot entry name :

STMN1_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of STMN-1. No significant cross-reactivity or interference between STMN-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between STMN-1 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This STMN-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human STMN-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Neurobiology

products description :

. Principle of the assay: STMN-1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for STMN-1. Standards or samples are then added to the microtiter plate wells and STMN-1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of STMN-1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for STMN-1 are added to each well to "sandwich" the STMN-1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain STMN-1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The STMN-1 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

197692597

ncbi acc num :

BAG70262.1

ncbi mol weight :

19,824 Da

ncbi pathways :

Aurora B Signaling Pathway (138080); Integrated Pancreatic Cancer Pathway (711360); MAPK Signaling Pathway (198779); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); MicroRNAs In Cancer Pathway (852705); MicroRNAs In Cancer Pathway (852928); RB In Cancer Pathway (920977); Regulation Of Microtubule Cytoskeleton Pathway (755439); Signaling Mediated By P38-gamma And P38-delta Pathway (138043)

ncbi summary :

This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]

uniprot summary :

STMN1: a microtubule-associated protein involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Phosphorylation reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, nonleukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia. Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 1p36.11. Cellular Component: microtubule; neuron projection; membrane; cytoplasm; intracellular; cytosol. Molecular Function: tubulin binding; signal transducer activity. Biological Process: mitotic spindle organization and biogenesis; axonogenesis; negative regulation of microtubule polymerization; response to virus; microtubule depolymerization; positive regulation of cell motility; brain development; signal transduction; neurite development; regulation of cytoskeleton organization and biogenesis

size1 :

48-Strip-Wells-(Competitive)

price1 :

470 USD

size2 :

48-Strip-Wells-(Sandwich)

price2 :

470

size3 :

96-Strip-Wells-(Competitive)

price3 :

675

size4 :

96-Strip-Wells-(Sandwich)

price4 :

675

more info or order :

MyBioSource product webpage