product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Rat Alanine Aminotransferase ELISA Kit
catalog :
MBS729318
quantity :
48-Strip-Wells
price :
440 USD
more info or order :
MyBioSource product webpage
image
image 1 :
MyBioSource MBS729318 image 1
product information
catalog number :
MBS729318
products type :
ELISA Kit
products full name :
Rat Alanine Aminotransferase ELISA Kit
products short name :
[Alanine Aminotransferase]
other names :
[ALT; ALT; activator of late transcription, experimental; ALT; ALT]
products gene name :
[ALT]
other gene names :
[TP901-1p29]
uniprot entry name :
Q9AZ68_9CAUD
reactivity :
Rat
specificity :
This assay has high sensitivity and excellent specificity for detection of ALT. No significant cross-reactivity or interference between ALT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ALT and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Detection Range: 1.0-25ng/mL. Sensitivity: 0.1ng/ml
products description :
Intended Uses: This ALT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ALT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: ALT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ALT antibody and an ALT-HRP conjugate. The assay sample and buffer are incubated together with ALT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALT concentration since ALT from samples and ALT-HRP conjugate compete for the anti-ALT antibody binding site. Since the number of sites is limited, as more sites are occupied by ALT from the sample, fewer sites are left to bind ALT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALT concentration in each sample is interpolated from this standard curve.
ncbi gi num :
13786560
ncbi acc num :
NP_112692.1
ncbi gb acc num :
NC_002747.1
ncbi mol weight :
16,731 Da
size1 :
48-Strip-Wells
price1 :
440 USD
size2 :
96-Strip-Wells
price2 :
640
size3 :
5x96-Strip-Wells
price3 :
2895
size4 :
10x96-Strip-Wells
price4 :
5415
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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