ELISA/assay

Mouse Basic Fibroldast Growth Factor ELISA Kit

MBS729312

48-Strip-Wells

470 USD

ELISA Kit

Mouse Basic Fibroldast Growth Factor ELISA Kit

Basic Fibroldast Growth Factor

Mouse Basic Fibroldast Growth Factor ELISA Kit; Basic Fibroldast Growth Factor; Basic Fibroldast Growth Factor ELISA Kit (Mouse)

basic fibroblast growth factor; Heparin-binding growth factor 2; heparin-binding growth factor 2; prostatropin; OTTHUMP00000037513; basic fibroblast growth factor bFGF; fibroblast growth factor 2 (basic); Basic fibroblast growth factor

BFGF

FGF2; FGF2; BFGF; FGFB; HBGF-2; FGFB

FGF2_HUMAN

Mouse

Sensitivity: The sensitivity in this assay is 1.0 pg/mL. Specificity: This assay has high sensitivity and excellent specificity for detection of BFGF. No significant cross-reactivity or interference between BFGF and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma

Mouse ELISA Kit

For Samples: Cell culture fluid & body fluid & tissue homogenate serum or blood plasma. Intended Uses: This BFGF ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of BFGF in the sample, this BFGF ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus BFGF concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of BFGF utilizes a polyclonal anti-BFGF antibody and an BFGF-HRP conjugate. The assay sample and buffer are incubated together with BFGF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BFGF concentration since BFGF from samples and BFGF-HRP conjugate compete for the anti-BFGF antibody binding site. Since the number of sites is limited, as more sites are occupied by BFGF from the sample, fewer sites are left to bind BFGF-HRP conjugate Standards of known BFGF concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (O.D.) to the concentration of BFGF. The BFGF concentration in each sample is interpolated from this standard curve.

8250666

P09038

30,770 Da

Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); Downstream Signaling Of Activated FGFR Pathway (160957); Endochondral Ossification Pathway (198812); FGFR Ligand Binding And Activation Pathway (106344); FGFR1 Ligand Binding And Activation Pathway (106345); FGFR1b Ligand Binding And Activation Pathway (106346); FGFR1c Ligand Binding And Activation Pathway (106347); FGFR2 Ligand Binding And Activation Pathway (106349); FGFR2c Ligand Binding And Activation Pathway (106351); FGFR3 Ligand Binding And Activation Pathway (106352)

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq]

FGF2: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2. Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue. Belongs to the heparin-binding growth factors family. 4 isoforms of the human protein are produced by alternative initiation. Protein type: Motility/polarity/chemotaxis; Activator. Chromosomal Location of Human Ortholog: 4q26. Cellular Component: extracellular space; extracellular region; nucleus. Molecular Function: heparin binding; protein binding; ligand-dependent nuclear receptor transcription coactivator activity; growth factor activity; cytokine activity; chemoattractant activity; fibroblast growth factor receptor binding. Biological Process: extracellular matrix organization and biogenesis; nerve growth factor receptor signaling pathway; wound healing; somatic stem cell maintenance; activation of MAPK activity; positive regulation of transcription, DNA-dependent; signal transduction; chemotaxis; hyaluronan catabolic process; growth factor dependent regulation of satellite cell proliferation; positive regulation of MAP kinase activity; positive chemotaxis; positive regulation of cell proliferation; embryonic morphogenesis; positive regulation of cardiac muscle cell proliferation; epidermal growth factor receptor signaling pathway; nervous system development; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; positive regulation of cell fate specification; positive regulation of blood vessel endothelial cell migration; positive regulation of phosphoinositide 3-kinase activity; negative regulation of blood vessel endothelial cell migration; regulation of angiogenesis; positive regulation of angiogenesis; organ morphogenesis; cell migration during sprouting angiogenesis; ureteric bud branching; positive regulation of cell division; release of sequestered calcium ion into cytosol; Ras protein signal transduction; insulin receptor signaling pathway; phosphatidylinositol biosynthetic process; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; inositol phosphate biosynthetic process

48-Strip-Wells

470 USD

96-Strip-Wells

675