Mouse Cartilage glycoprotein 39/YKL40 ELISA Kit | MyBioSource MBS729256 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Cartilage glycoprotein 39/YKL40 ELISA Kit

catalog :

MBS729256

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS729256

products type :

ELISA Kit

products full name :

Mouse Cartilage glycoprotein 39/YKL40 ELISA Kit

products short name :

Cartilage glycoprotein 39/YKL40

other names :

chitinase-3-like protein 1; Chitinase-3-like protein 1; chitinase-3-like protein 1; 39 kDa synovial protein; cartilage glycoprotein 39; chitinase 3-like 1 (cartilage glycoprotein-39); 39 kDa synovial protein; Cartilage glycoprotein 39; CGP-39; GP-39; hCGP-39; YKL-40

products gene name :

YKL40

other gene names :

CHI3L1; CHI3L1; GP39; ASRT7; GP-39; YKL40; CGP-39; YKL-40; YYL-40; HC-gp39; HCGP-3P; hCGP-39; CGP-39; GP-39; hCGP-39

uniprot entry name :

CH3L1_HUMAN

reactivity :

Mouse

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This YKL40 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse YKL40. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Immunology

products description :

Principle of the Assay: YKL40 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for YKL40. Standards or samples are then added to the microtiter plate wells and YKL40 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of YKL40 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for YKL40 are added to each well to "sandwich" the YKL40 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain YKL40 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The YKL40 concentration in each sample is interpolated from this standard curve

ncbi gi num :

144226251

ncbi acc num :

NP_001267.2

ncbi gb acc num :

NM_001276.2

uniprot acc num :

P36222

ncbi mol weight :

42,625 Da

ncbi pathways :

Amino Sugar And Nucleotide Sugar Metabolism Pathway (82979); Amino Sugar And Nucleotide Sugar Metabolism Pathway (350)

ncbi summary :

Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling. [provided by RefSeq, Sep 2009]

uniprot summary :

CHI3L1: Carbohydrate-binding lectin with a preference for chitin. May play a role in defense against pathogens, or in tissue remodeling. May play an important role in the capacity of cells to respond to and cope with changes in their environment. A genetic variation in CHI3L1 is associated with susceptibility to asthma-related traits type 7 (ASRT7). Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing and dyspnea, bronchial hyperresponsiveness (BHR) as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis. Belongs to the glycosyl hydrolase 18 family. Protein type: Secreted, signal peptide; Cell adhesion; Secreted. Chromosomal Location of Human Ortholog: 1q32.1. Cellular Component: proteinaceous extracellular matrix; extracellular space; endoplasmic reticulum; perinuclear region of cytoplasm; cytoplasm. Molecular Function: extracellular matrix structural constituent; chitin binding; chitinase activity; carbohydrate binding. Biological Process: positive regulation of protein kinase B signaling cascade; positive regulation of angiogenesis; response to mechanical stimulus; apoptosis; cartilage development; carbohydrate metabolic process; activation of NF-kappaB-inducing kinase; immune response; chitin catabolic process; inflammatory response; lung development. Disease: Schizophrenia; Asthma-related Traits, Susceptibility To, 7

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage