ELISA/assay

Human Prolactin ELISA Kit

MBS729243

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human Prolactin ELISA Kit

Prolactin

Prolactin; Prolactin; prolactin; decidual prolactin; prolactin

PRL

PRL; PRL; PRL

PRL_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of PRL. No significant cross-reactivity or interference between PRL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PRL and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 ng/mL.

Intended Uses: This PRL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PRL. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: PRL ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PRL antibody and an PRL-HRP conjugate. The assay sample and buffer are incubated together with PRL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PRL concentration since PRL from samples and PRL-HRP conjugate compete for the anti-PRL antibody binding site. Since the number of sites is limited, as more sites are occupied by PRL from the sample, fewer sites are left to bind PRL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRL concentration in each sample is interpolated from this standard curve.

130930

P01236.1

25,876 Da

Amyloids Pathway (366238); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Disease Pathway (530764); ErbB4 Signaling Events Pathway (137991); Glucocorticoid Receptor Regulatory Network Pathway (138014); Growth Hormone Receptor Signaling Pathway (477128); Immune System Pathway (106386); Jak-STAT Signaling Pathway (83077)

This gene encodes the anterior pituitary hormone prolactin. This secreted hormone is a growth regulator for many tissues, including cells of the immune system. It may also play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]

prolactin: Prolactin acts primarily on the mammary gland by promoting lactation. Belongs to the somatotropin/prolactin family. Protein type: Cytokine; Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 6p22.3. Cellular Component: extracellular region. Molecular Function: protein binding; prolactin receptor binding; hormone activity. Biological Process: lactation; cell proliferation; cell surface receptor linked signal transduction; regulation of multicellular organism growth; positive regulation of JAK-STAT cascade; female pregnancy

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675