catalog number :
MBS729223
products type :
ELISA Kit
products full name :
Rat alpha2 antiplasmin ELISA Kit
products short name :
alpha2 antiplasmin
products name syn :
Rat alpha2 antiplasmin ELISA Kit; alpha2 antiplasmin; a2 antiplasmin; 2 antiplasmin; alpha2 antiplasmin (Rat)
other names :
alpha-2-antiplasmin; alpha-2-antiplasmin; alpha-2-plasmin inhibitor; alpha-2-PI serine protease inhibitor
products gene name :
alpha2-AP
products gene name syn :
a2-AP; 2-AP
other gene names :
SERPINF2; serpin
specificity :
Sensitivity: The sensitivity in this assay is 0.1 ng/mL. Specificity: This assay has high sensitivity and excellent specificity for detection of A2-AP. No significant cross-reactivity or interference between A2-AP and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
other info2 :
Intended Uses: This APalpha2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat APalpha2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Rat ELISA Kit
products description :
Principle of the Assay: APalpha2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-APalpha2 antibody and an APalpha2-HRP conjugate. The assay sample and buffer are incubated together with APalpha2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the APalpha2 concentration since APalpha2 from samples and APalpha2-HRP conjugate compete for the anti-APalpha2 antibody binding site. Since the number of sites is limited, as more sites are occupied by APalpha2 from the sample, fewer sites are left to bind APalpha2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The APalpha2 concentration in each sample is interpolated from this standard curve.
ncbi acc num :
NP_001076210.1
ncbi gb acc num :
NM_001082741.2