ELISA/assay

Rat Peroxisome Proliferators activator alpha ELISA Kit

MBS729154

48-Strip-Wells

470 USD

ELISA Kit

Rat Peroxisome Proliferators activator alpha ELISA Kit

Peroxisome Proliferators activator alpha

Rat Peroxisome Proliferators activator a ELISA Kit

PPAR-alpha

PPAR-a

Rat

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture superna tants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This PPAa ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat PPAa. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY PPAa ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PPAa antibody and an PPAa-HRP conjugate. The assay sample and buffer are incubated together with PPAa-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubatedwith a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PPAa concentration since PPAa from samples and PPAa-HRP conjugate compete for the anti-PPAa antibody binding site. Since the number of sites is limited, as more sites are occupied by PPAa from the sample, fewer sites are left to bind PPAa-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PPAa concentration in each sample is interpolated from this standard curve.

48-Strip-Wells

470 USD

96-Strip-Wells

675