ELISA/assay

Rat Granzymes B ELISA Kit

MBS729150

48-Strip-Wells

470 USD

ELISA Kit

Rat Granzymes B ELISA Kit

Granzymes B

granzyme B; Granzyme B; granzyme B; C11; CTLA-1; fragmentin 2; fragmentin-2; cathepsin G-like 1; human lymphocyte protein; T-cell serine protease 1-3E; cytotoxic serine protease B; cytotoxic T-lymphocyte proteinase 2; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); C11; CTLA-1; Cathepsin G-like 1; CTSGL1; Cytotoxic T-lymphocyte proteinase 2; Lymphocyte protease; Fragmentin-2; Granzyme-2; Human lymphocyte protein; HLP; SECT; T-cell serine protease 1-3E

Gzms-B

GZMB; GZMB; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1; CGL1; CSPB; CTLA1; GRB; CTSGL1; Lymphocyte protease; HLP

GRAB_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of GMB. No significant cross-reactivity or interference between GMB and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GMB and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Immunology

Intended Uses This GMB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat GMB. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: GMB ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GMB antibody and an GMB-HRP conjugate. The assay sample and buffer are incubated together with GMB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GMB concentration since GMB from samples and GMB-HRP conjugate compete for the anti-GMB antibody binding site. Since the number of sites is limited, as more sites are occupied by GMB from the sample, fewer sites are left to bind GMB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GMB concentration in each sample is interpolated from this standard curve.

221625528

NP_004122.2

NM_004131.4

27,716 Da

Activation, Myristolyation Of BID And Translocation To Mitochondria Pathway (105657); Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Apoptosis Pathway (198797); Apoptosis Pathway (105648); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); Caspase Cascade In Apoptosis Pathway (137974); Downstream Signaling In Naive CD8+ T Cells Pathway (138018)

Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response. [provided by RefSeq, Jul 2008]

GZMB: This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. By staphylococcal enterotoxin A (SEA) in peripheral blood leukocytes. Inactivated by the serine protease inhibitor diisopropylfluorophosphate. Belongs to the peptidase S1 family. Granzyme subfamily. Protein type: EC 3.4.21.79; Apoptosis; Protease. Chromosomal Location of Human Ortholog: 14q11.2. Cellular Component: intracellular membrane-bound organelle; membrane; cytoplasm; immunological synapse; cytosol; nucleus. Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity. Biological Process: Notch signaling pathway; induction of apoptosis by granzyme; apoptosis; natural killer cell mediated cytotoxicity; cytolysis; protein processing; immune response; proteolysis

48-Strip-Wells

470 USD

96-Strip-Wells

675