ELISA/assay

Human Basic leucine zipper transcription factor ATF like ELISA Kit

MBS729096

48-Strip-Wells

470 USD

ELISA Kit

Human Basic leucine zipper transcription factor ATF like ELISA Kit

Basic leucine zipper transcription factor ATF like

basic leucine zipper transcription factor, ATF-like; Basic leucine zipper transcriptional factor ATF-like; basic leucine zipper transcriptional factor ATF-like; SF-HT-activated gene 2 protein; activating transcription factor B; B-cell-activating transcription factor; basic leucine zipper transcription factor, ATF-like; B-cell-activating transcription factor; B-ATF; SF-HT-activated gene 2 protein; SFA-2

BATF

BATF; BATF; SFA2; B-ATF; BATF1; SFA-2; B-ATF; SFA-2

BATF_HUMAN

Human

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture superna tants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.

Epigenetics and Nuclear Signaling

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This BLZTF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human BLZTF. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY BLZTF ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for BLZTF. Standards or samples are then added to the microtiter plate wells and BLZTF if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of BLZTF present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for BLZTF are added to each well to “sandwich” the BLZTF immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain BLZTF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BLZTF concentration in each sample is interpolated from this standard curve.

119601640

EAW81234.1

14,120 Da

The protein encoded by this gene is a nuclear basic leucine zipper protein that belongs to the AP-1/ATF superfamily of transcription factors. The leucine zipper of this protein mediates dimerization with members of the Jun family of proteins. This protein is thought to be a negative regulator of AP-1/ATF transcriptional events. [provided by RefSeq, Jul 2008]

BATF: a nuclear basic leucine zipper protein that belongs to the AP-1/ATF superfamily of transcription factors. Functions as a negative regulator of AP-1 mediated transcription by binding to Jun proteins. Jun/B-ATF heterodimers bind DNA preferentially at the 12-O-tetradecanoylphorbol-13- acetate response element (TRE) and weaker at the cAMP responsive region (CRE), but are transcriptionally inert. Protein type: Transcription factor; DNA-binding. Chromosomal Location of Human Ortholog: 14q24.3. Cellular Component: cytoplasm; nucleus. Molecular Function: protein binding; sequence-specific DNA binding; transcription factor activity. Biological Process: regulation of transcription from RNA polymerase II promoter; DNA damage response, signal transduction by p53 class mediator; transcription, DNA-dependent; isotype switching; cytokine production; myeloid dendritic cell differentiation; lymphoid progenitor cell differentiation; T-helper 2 cell differentiation; defense response to protozoan; response to DNA damage stimulus

48-Strip-Wells

470 USD

96-Strip-Wells

675