catalog number :
MBS729029
products type :
ELISA Kit
products full name :
Mouse Synaptosome Associated Protein 25 ELISA Kit
products short name :
Synaptosome Associated Protein 25
other names :
SNAP-25; SNAP-25
products gene name :
SNAP-25
other gene names :
LOC100533400
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich
other info2 :
Intended Uses: This SNAP-25 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse SNAP-25. This ELISA kit for research use only, not for therapeutic or diagnostic applications. !Intended Uses: This SNAP-25 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse SNAP-25. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Neurobiology
products description :
Principle of the Assay: SNAP-25 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SNAP-25. Standards or samples are then added to the microtiter plate wells and SNAP-25 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SNAP-25 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for SNAP-25 are added to each well to "sandwich" the SNAP-25 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SNAP-25 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SNAP-25 concentration in each sample is interpolated from this standard curve.
ncbi acc num :
NP_001191612.1
ncbi gb acc num :
NM_001204683.1