ELISA/assay

Rat Matrix Metalloproteinase 3 ELISA Kit

MBS729026

48-Strip-Wells

440 USD

ELISA Kit

Rat Matrix Metalloproteinase 3 ELISA Kit

[Matrix Metalloproteinase 3]

[MMP-3, partial; MMP-3; stromelysin-1; metalloproteinase 3 receptor; matrix metallopeptidase 3 (stromelysin 1, progelatinase)<; MMP-3]

[MMP3]

[MMP3; bmmp-3]

Q9N283_BOVIN

Rat

The microtiter plates are coated with murine monoclonal antibodies against human MMP-3. Cross reactivities with other antigens could not be detected.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Human Serum. Sensitivity: 5 ng/ml.

Cancer

Intended Uses: This MMP-3 ELISA is a solid phase enzyme immunoassay with two different monoclonal antibodies against human MMP-3 for the precise measurement of MMP-3 concentration in human serum. For research use only, not for use in diagnostic procedures. Principle of the Assay: The 1:10 diluted serum samples are incubated in cavities which are coated with a monoclonal antihuman MMP-3 antibody. In this process, MMP-3 from the sample serum binds to the antibody on the plate; unbound serum components are washed away in the subsequent washing step. A monoclonal anti-human MMP-3 antibody, which is marked with horseradish peroxidase (conjugate), is then added. During incubation, this binds to the previously formed antibody-MMP3-complex. Unbound conjugate is removed in the subsequent washing step. Bonded MMP-3 is detected by an enzymatic color reaction (blue) of the substrate, which is stopped using diluted acid (sudden color change to yellow). The intensity of the color development of the chromogen depends on the amount of conjugate bound to the antibody-MMP-3-complex and is therefore directly proportional to the MMP-3 concentration in the serum!!Background/Introduction: Rheumatoid arthritis (RA) is a chronic inflammatory disease which is primarily characterised by joint inflammation and leads to a loss of joint function and severe disability if the progress of the disease is not tackled in time. Matrix metalloproteinase-3 (MMP-3, stromelysin-1) belongs to the family of metalloproteinases. It is predominantly expressed by connective tissue cells and plays an important role in the material conversion processes of the extracellular matrix (ECM). MMP-3 can break down many components of the ECM such as proteoglycans, fibronectin, laminin and various other collagens. As a result of this large range of substrates and the ability to activate other degrading enzymes, including other matrix metalloproteinases, MMP-3 plays a key role in the physiological and pathological processes of tissue remodelling. MMP-3 is secreted in an inactive form (pro-MMP-3, 52 kDa) and activated by limited proteolysis by endopeptidases. Active MMP-3 (45 kDa and 35 kDa) can be inhibited as a result of binding to tissue inhibitors of matrix metalloproteinases (TIMP) or alpha2-macroglobulin. Regulating MMP-3 activity is necessary to prevent the destruction of the ECM and to preserve the physiological equilibrium in tissue remodelling processes. Accordingly, individuals with various pathological conditions such as cancer, atherosclerosis and arthritis have elevated MMP-3 concentrations.

8176609

BAA96388.1

16,673 Da

Matrix Metalloproteinases Pathway (198565); Rheumatoid Arthritis Pathway (200308); Rheumatoid Arthritis Pathway (200269); TNF Signaling Pathway (812258); TNF Signaling Pathway (813210); Transcriptional Misregulation In Cancer Pathway (522997); Transcriptional Misregulation In Cancer Pathway (522987)

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415