ELISA/assay

Mouse S100 Calcium Binding Protein A12 ELISA Kit

MBS728991

48-Strip-Wells

470 USD

ELISA Kit

Mouse S100 Calcium Binding Protein A12 ELISA Kit

S100 Calcium Binding Protein A12

S100 calcium binding protein A12 (calgranulin C); Protein S100-A12; protein S100-A12; EN-RAGE; calgranulin C; calgranulin-C; neutrophil S100 protein; calcium-binding protein in amniotic fluid 1; migration inhibitory factor-related protein 6; S100 calcium-binding protein A12 (calgranulin C); extracellular newly identified RAGE-binding protein; S100 calcium binding protein A12; CGRP; Calcium-binding protein in amniotic fluid 1; CAAF1; Calgranulin-C; CAGC; Extracellular newly identified RAGE-binding protein; EN-RAGE; Migration inhibitory factor-related protein 6; MRP-6; p6; Neutrophil S100 protein; S100 calcium-binding protein A12Cleaved into the following chain:Calcitermin

S100A12

S100A12; S100A12; p6; CAGC; CGRP; MRP6; CAAF1; MRP-6; ENRAGE; CAAF1; CAGC; EN-RAGE; MRP-6; p6

S10AC_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of S100A12. No significant cross-reactivity or interference between S100A12 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between S100A12 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.

Signal Transduction

Intended Uses: This S100A12 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse S100A12. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay S100A12 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for S100A12. Standards or samples are then added to the microtiter plate wells and S100A12 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of S100A12 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for S100A12 are added to each well to "sandwich" the S100A12 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain S100A12 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The S100A12 concentration in each sample is interpolated from this standard curve.

119573717

EAW53332.1

10,575 Da

Activated TLR4 Signalling Pathway (106400); Advanced Glycosylation Endproduct Receptor Signaling Pathway (187092); Cytosolic Sensors Of Pathogen-associated DNA Pathway (576255); DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway (833822); Immune System Pathway (106386); Innate Immune System Pathway (106387); MyD88 Cascade Initiated On Plasma Membrane Pathway (205107); MyD88 Dependent Cascade Initiated On Endosome Pathway (187081); MyD88-independent Cascade Pathway (106401); MyD88:Mal Cascade Initiated On Plasma Membrane Pathway (106390)

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities. [provided by RefSeq, Jul 2008]

S100A12: S100A12 is a calcium-, zinc- and copper-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. Its proinflammatory activity involves recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to receptor for advanced glycation endproducts (AGER). Binding to AGER activates the MAP-kinase and NF-kappa-B signaling pathways leading to production of proinflammatory cytokines and up-regulation of cell adhesion molecules ICAM1 and VCAM1. Acts as a monocyte and mast cell chemoattractant. Can stimulate mast cell degranulation and activation which generates chemokines, histamine and cytokines inducing further leukocyte recruitment to the sites of inflammation. Can inhibit the activity of matrix metalloproteinases; MMP2, MMP3 and MMP9 by chelating Zn(2+) from their active sites. Possesses filariacidal and filariastatic activity. Calcitermin possesses antifungal activity against C.albicans and is also active against E.coli and P.aeruginosa but not L.monocytogenes and S.aureus. Belongs to the S-101 family. Chromosomal Location of Human Ortholog: 1q21. Cellular Component: cytoskeleton; cytoplasm; extracellular region; plasma membrane; cytosol; nucleus. Molecular Function: protein binding; RAGE receptor binding; copper ion binding; zinc ion binding; calcium ion binding. Biological Process: neutrophil chemotaxis; positive regulation of I-kappaB kinase/NF-kappaB cascade; killing of cells of another organism; mast cell activation; activation of NF-kappaB transcription factor; monocyte chemotaxis; positive regulation of MAP kinase activity; xenobiotic metabolic process; defense response to bacterium; innate immune response; inflammatory response; defense response to fungus; cytokine secretion; positive regulation of inflammatory response

48-Strip-Wells

470 USD

96-Strip-Wells

675