ELISA/assay

Rat Protein C ELISA Kit

MBS728974

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Rat Protein C ELISA Kit

Protein C

proC; proC; hypothetical protein

ProC

proC

Rat

This assay has high sensitivity and excellent specificity for detection of PC. No significant cross-reactivity or interference between PC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PC and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 mug/mL.

Intended Uses: This PC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat PC. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the assay: PC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PC antibody and an PC-HRP conjugate. The assay sample and buffer are incubated together with PC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PC concentration since PC from samples and PC-HRP conjugate compete for the anti-PC antibody binding site. Since the number of sites is limited, as more sites are occupied by PC from the sample, fewer sites are left to bind PC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PC concentration in each sample is interpolated from this standard curve.

147829653

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48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675