catalog number :
MBS728899
products type :
ELISA Kit
products full name :
Human Pancreatic Derived Factor/FAM3B Antibody ELISA Kit
products short name :
Pancreatic Derived Factor/FAM3B Antibody
products gene name :
PANDER
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.
products categories :
Immunology
products description :
For Sample Serum, plasma, cell culture supernatants, body fluid and tissue homogenate INTENDED USE: This anti-PANDER ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human anti-PANDER. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY: anti-PANDER ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with Pancreatic-Derived Factor/FAM3B Antigen (anti-PANDER). Standards or samples are then added to the microtiter plate wells and anti-PANDER if present, will bind to the antigen pre-coated wells. In order to quantitatively determine the amount of anti-PANDER present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated Pancreatic-Derived Factor/FAM3B Antigen (anti-PANDER) are added to each well to “sandwich” the anti-PANDER immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain anti-PANDER and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The anti-PANDER concentration in each sample is interpolated from this standard curve.
size1 :
48-Strip-Wells-(Competitive)
size2 :
48-Strip-Wells-(Sandwich)
size3 :
96-Strip-Wells-(Competitive)
size4 :
96-Strip-Wells-(Sandwich)