catalog number :
MBS728851
products type :
ELISA Kit
products full name :
Human Anti Melanoma associated antigen ELISA Kit
products short name :
Anti Melanoma associated antigen
other names :
MAGE; MAGE; CG10059 gene product from transcript CG10059-RA; CG10059-PA; MAGE; MAGE-PA; necdin; sleepless in seattle; MAGE; RE25453p
products gene name :
Anti MAA
other gene names :
MAGE; MAGE; CG10059; DMAGE; DmelCG10059; Nse3; sst; CG10059; Dmel_CG10059
uniprot entry name :
Q9I7L4_DROME
storage stability :
Store all reagents at 2-8 degree C.
products categories :
Cancer
products description :
For Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This BG MAGE ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human MAGE. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: MAGE ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MAGE antibody and an MAGE-HRP conjugate. The assay sample and buffer are incubated together with MAGE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MAGE concentration since MAGE from samples and MAGE-HRP conjugate compete for the anti-MAGE antibody binding site. Since the number of sites is limited, as more sites are occupied by MAGE from the sample, fewer sites are left to bind MAGE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MAGE concentration in each sample is interpolated from this standard curve.
ncbi acc num :
NP_649702.2
ncbi gb acc num :
NM_141445.3
ncbi mol weight :
26,457 Da
ncbi pathways :
Apoptosis Pathway 1005319!!Regulation Of Apoptosis Pathway 1005330!!Role Of DCC In Regulating Apoptosis Pathway 1005333