ELISA/assay

Rat Netrin-1 ELISA Kit

MBS728764

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Rat Netrin-1 ELISA Kit

Netrin-1

netrin-1; Netrin-1; netrin-1; netrin 1, mouse, homolog of; epididymis tissue protein Li 131P; netrin 1; Epididymis tissue protein Li 131P

Ntn1

NTN1; NTN1; NTN1L; NTN1L

NET1_HUMAN

Rat

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 pg/mL.

Neurobiology

Intended Uses: This NTn1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat NTn1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: NTn1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NTn1 antibody and an NTn1-HRP conjugate. The assay sample and buffer are incubated together with NTn1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NTn1 concentration since NTn1 from samples and NTn1-HRP conjugate compete for the anti-NTn1 antibody binding site. Since the number of sites is limited, as more sites are occupied by NTn1 from the sample, fewer sites are left to bind NTn1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NTn1 concentration in each sample is interpolated from this standard curve.

148613884

NP_004813.2

NM_004822.2

67,748 Da

Alpha6-Beta4 Integrin Signaling Pathway (198807); Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688); Cell-Cell Communication Pathway (477132); DCC Mediated Attractive Signaling Pathway (161000); DSCAM Interactions Pathway (187229); Developmental Biology Pathway (477129); Netrin Mediated Repulsion Signals Pathway (161001); Netrin-1 Signaling Pathway (160999)

Netrin is included in a family of laminin-related secreted proteins. The function of this gene has not yet been defined; however, netrin is thought to be involved in axon guidance and cell migration during development. Mutations and loss of expression of netrin suggest that variation in netrin may be involved in cancer development. [provided by RefSeq, Jul 2008]

NTN1: Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in tumorigenesis by regulating apoptosis. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 17p13.1. Cellular Component: cytoplasm; extracellular region; basement membrane. Molecular Function: protein binding. Biological Process: axon guidance; inner ear morphogenesis; cell-cell adhesion; establishment of nucleus localization; apoptosis; positive regulation of axon extension; positive regulation of cell proliferation; neuron migration; negative regulation of axon extension; regulation of cell migration; anterior/posterior axon guidance

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675