catalog number :
MBS728719
products type :
ELISA Kit
products full name :
Human Ischemia Modified Albumin ELISA Kit
products short name :
[Ischemia Modified Albumin]
products gene name :
[IMA]
specificity :
This assay has high sensitivity and excellent specificity for detection of IL-8. No significant cross-reactivity or interference between IL-8 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-8 and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 1.0 pg/mL.
products categories :
Cardiovascular
products description :
Intended Uses: This IL-8 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep IL-8. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: IL-8 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IL-8 antibody and an IL-8-HRP conjugate. The assay sample and buffer are incubated together with IL-8-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-8 concentration since IL-8 from samples and IL-8-HRP conjugate compete for the anti-IL-8 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-8 from the sample, fewer sites are left to bind IL-8-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-8 concentration in each sample is interpolated from this standard curve.
size1 :
48-Strip-Wells-(Competitive)
size2 :
48-Strip-Wells-(Sandwich)
size3 :
96-Strip-Wells-(Competitive)
size4 :
96-Strip-Wells-(Sandwich)
size5 :
5x96-Strip-Wells-(Competitive)
size6 :
5x96-Strip-Wells-(Sandwich)
size7 :
10x96-Strip-Wells-(Competitive)
size8 :
10x96-Strip-Wells-(Sandwich)