ELISA/assay

Rat Metallothionein 2 ELISA Kit

MBS728702

48-Strip-Wells

470 USD

ELISA Kit

Rat Metallothionein 2 ELISA Kit

Metallothionein 2

metallothionein-2; Metallothionein-2; metallothionein-2; MT-2; MT-II; metallothionein-2A; metallothionein-II; metallothionein 2A; Metallothionein-2A; Metallothionein-II; MT-II

MT2

MT2A; MT2A; MT2; CES1; MT2; MT-2; MT-II

MT2_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of MT. No significant cross-reactivity or interference between MT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MT and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This MT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat MT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Microbiology

Principle of the assay: MT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MT antibody and an MT-HRP conjugate. The assay sample and buffer are incubated together with MT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MT concentration since MT from samples and MT-HRP conjugate compete for the anti-MT antibody binding site. Since the number of sites is limited, as more sites are occupied by MT from the sample, fewer sites are left to bind MT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MT concentration in each sample is interpolated from this standard curve.

5174764

NP_005944.1

NM_005953.3

6,042 Da

Cytokine Signaling In Immune System Pathway (366171); Immune System Pathway (106386); Interferon Signaling Pathway (187103); Interferon Gamma Signaling Pathway (187106); Mineral Absorption Pathway (212237); Mineral Absorption Pathway (212220)

MT2A: Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids. Belongs to the metallothionein superfamily. Type 1 family. Protein type: Carrier. Chromosomal Location of Human Ortholog: 16q13. Cellular Component: perinuclear region of cytoplasm; cytoplasm; cytosol; nucleus. Molecular Function: protein binding; zinc ion binding; drug binding. Biological Process: cellular copper ion homeostasis; cytokine and chemokine mediated signaling pathway; negative regulation of growth

48-Strip-Wells

470 USD

96-Strip-Wells

675