ELISA/assay

Human Placental Alkaline Phosphatase ELISA Kit

MBS728565

48-Strip-Wells

470 USD

ELISA Kit

Human Placental Alkaline Phosphatase ELISA Kit

Placental Alkaline Phosphatase

placental alkaline phosphatase; Alkaline phosphatase, placental type; alkaline phosphatase, placental type; Regan isozyme; glycerophosphatase; alkaline phosphomonoesterase; placental alkaline phosphatase 1; alkaline phosphatase Regan isozyme; alkaline phosphatase, placental; Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1; PLAP-1

PLAP

ALPP; ALPP; ALP; PALP; PLAP; PLAP-1; PLAP; PLAP-1

PPB1_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of ALPP. No significant cross-reactivity or interference between ALPP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ALPP and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Quantitative Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This ALPP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ALPP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: ALPP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ALPP antibody and an ALPP-HRP conjugate. The assay sample and buffer are incubated together with ALPP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALPP concentration since ALPP from samples and ALPP-HRP conjugate compete for the anti-ALPP antibody binding site. Since the number of sites is limited, as more sites are occupied by ALPP from the sample, fewer sites are left to bind ALPP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALPP concentration in each sample is interpolated from this standard curve.

178464

AAA51706.1

57,954 Da

Folate Biosynthesis Pathway (83018); Folate Biosynthesis Pathway (404); Metabolic Pathways (132956)

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. [provided by RefSeq, Jul 2008]

ALPP: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3 untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. [provided by RefSeq, Jul 2008]. Protein type: EC 3.1.3.1; Cell surface; Motility/polarity/chemotaxis; Membrane protein, integral; Cofactor and Vitamin Metabolism - folate biosynthesis; Membrane protein, GPI anchor; Phosphatase (non-protein). Chromosomal Location of Human Ortholog: 2q37. Cellular Component: cell surface; plasma membrane; integral to membrane. Molecular Function: zinc ion binding; alkaline phosphatase activity; magnesium ion binding. Biological Process: dephosphorylation

48-Strip-Wells

470 USD

96-Strip-Wells

675