Rat Follistatin ELISA Kit | MyBioSource MBS728531 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Follistatin ELISA Kit

catalog :

MBS728531

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS728531

products type :

ELISA Kit

products full name :

Rat Follistatin ELISA Kit

products short name :

Follistatin

other names :

follistatin isoform 2; Follistatin; follistatin; activin-binding protein; follistatin; Activin-binding protein

products gene name :

other gene names :

Fst; Fst; FS; AL033346; FS

uniprot entry name :

FST_MOUSE

reactivity :

Rat

specificity :

This assay has high sensitivity and excellent specificity for detection of FS. No significant cross-reactivity or interference between FS and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FS and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This FS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat FS. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Signal Transduction

products description :

. Principle of the assay: FS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FS antibody and an FS-HRP conjugate. The assay sample and buffer are incubated together with FS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FS concentration since FS from samples and FS-HRP conjugate compete for the anti-FS antibody binding site. Since the number of sites is limited, as more sites are occupied by FS from the sample, fewer sites are left to bind FS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FS concentration in each sample is interpolated from this standard curve.

ncbi gi num :

6679867

ncbi acc num :

NP_032072.1

ncbi gb acc num :

NM_008046.3

ncbi mol weight :

37,866 Da

ncbi pathways :

Antagonism Of Activin By Follistatin Pathway (1000997); Signal Transduction Pathway (970540); Signaling By Activin Pathway (1000996); TGF Beta Signaling Pathway (198393); TGF-beta Signaling Pathway (83261); TGF-beta Signaling Pathway (475)

ncbi summary :

The protein encoded by this gene binds to and negatively regulates activin, as well as other members of the transforming growth factor beta family, and acts to prevent uncontrolled cellular proliferation. This protein also contains a heparin-binding sequence. It is expressed in many of the tissues in which activin is synthesized and is thought to clear activin from the circulation by attachment to the cell surface. Alternative splicing results in multiple transcript variants that encode multiple protein isoforms, including FST315 and FST288, that differ at their C-terminus. Another isoform, FST303 is thought to be produced by proteolytic cleavage of FST315. These isoforms differ in their localization and in their ability to bind heparin. While FST315 is a circulating protein, FST288 is tissue-bound, and FST303 is gonad-specific. While deletion of all isoforms results in embryonic lethality, expression of just FST288 is sufficient for embryonic development, but the resultant mice have fertility defects. [provided by RefSeq, Aug 2014]

uniprot summary :

FST: Binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH). Protein type: Secreted; Secreted, signal peptide. Cellular Component: cytoplasm; extracellular region; nucleus. Molecular Function: heparan sulfate proteoglycan binding; activin binding. Biological Process: BMP signaling pathway; positive regulation of hair follicle development; negative regulation of activin receptor signaling pathway; negative regulation of cell differentiation; hair follicle morphogenesis; gamete generation; hemopoietic progenitor cell differentiation; keratinocyte proliferation; negative regulation of transcription from RNA polymerase II promoter; skeletal development; pattern specification process; female gonad development; odontogenesis of dentine-containing teeth

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage