ELISA/assay

Guinea pig Aggrecan ELISA Kit

MBS728530

48-Strip-Wells

470 USD

ELISA Kit

Guinea pig Aggrecan ELISA Kit

Aggrecan

aggrecan core protein; Aggrecan core protein; aggrecan core protein; CSPCP; aggrecan 1; cartilage-specific proteoglycan core protein; aggrecan, structural proteoglycan of cartilage; aggrecan; Cartilage-specific proteoglycan core protein; CSPCP

AGC

Acan; Acan; Agc; Agc1; Agc; Agc1; CSPCP

PGCA_RAT

Guinea Pig

This assay has high sensitivity and excellent specificity for detection of AGC. No significant cross-reactivity or interference between AGC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AGC and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This AGC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig AGC. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Priciple of the assay: AGC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AGC antibody and an AGC-HRP conjugate. The assay sample and buffer are incubated together with AGC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AGC concentration since AGC from samples and AGC-HRP conjugate compete for the anti-AGC antibody binding site. Since the number of sites is limited, as more sites are occupied by AGC from the sample, fewer sites are left to bind AGC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGC concentration in each sample is interpolated from this standard curve.

11990616

NP_071526.1

NM_022190.1

225,171 Da

Endochondral Ossification Pathway (219771); Spinal Cord Injury Pathway (739006)

an extracellular matrix proteoglycan; cleaved by ADAMTS1 [RGD, Feb 2006]

48-Strip-Wells

470 USD

96-Strip-Wells

675