ELISA/assay

Human Interferon Alpha ELISA Kit

MBS728432

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human Interferon Alpha ELISA Kit

Interferon Alpha

interferon-alpha; Interferon alpha-8; interferon alpha-8; leIF B; IFN-alpha-8; interferon alpha-B; interferon alpha-B'; interferon alpha-B2; interferon alpha type 201; interferon, alpha 8; Interferon alpha-B; LeIF B; Interferon alpha-B2

IFN Alpha

IFNA8; IFNA8; IFN-alphaB; IFN-alpha-8; LeIF B

IFNA8_HUMAN

Human

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 pg/mL.

Intended Uses: This alpha IFN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human alpha IFN. This ELISA kit for research use only, not for therapeutic or diagnostic applications

Cancer

Principle of the Assay: alpha IFN ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for alpha IFN. Standards or samples are then added to the microtiter plate wells and alpha IFN if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of alpha IFN present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for alpha IFN are added to each well to "sandwich" the alpha IFN immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain alpha IFN and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The alpha IFN concentration in each sample is interpolated from this standard curve.

306902

AAA52716.1

21,989 Da

Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833); Factors Involved In Megakaryocyte Development And Platelet Production Pathway (187196); Hemostasis Pathway (106028); Hepatitis B Pathway (694606)

IFNA8: Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. Belongs to the alpha/beta interferon family. Protein type: Secreted, signal peptide; Secreted; Cytokine. Chromosomal Location of Human Ortholog: 9p22. Cellular Component: extracellular space; extracellular region. Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interferon-alpha/beta receptor binding; cytokine activity. Biological Process: B cell proliferation; adaptive immune response; response to exogenous dsRNA; cytokine and chemokine mediated signaling pathway; B cell differentiation; regulation of MHC class I biosynthetic process; natural killer cell activation during immune response; innate immune response; blood coagulation; defense response to virus; humoral immune response; T cell activation during immune response; positive regulation of peptidyl-serine phosphorylation of STAT protein

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675