ELISA/assay

Guinea pig CXC-chemokine ligand 16 ELISA Kit

MBS728413

48-Strip-Wells

470 USD

ELISA Kit

Guinea pig CXC-chemokine ligand 16 ELISA Kit

CXC-chemokine ligand 16

C-X-C motif chemokine 16; C-X-C motif chemokine 16; C-X-C motif chemokine 16; CXC chemokine ligand 16; small-inducible cytokine B16; transmembrane chemokine CXCL16; scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; chemokine (C-X-C motif) ligand 16; Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; SR-PSOX; Small-inducible cytokine B16; Transmembrane chemokine CXCL16

CXCL16

CXCL16; CXCL16; SRPSOX; CXCLG16; SR-PSOX; SCYB16; SRPSOX; UNQ2759/PRO6714; SR-PSOX

CXL16_HUMAN

Guinea Pig

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Immunology

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This CXCL16 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig CXCL16. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY CXCL16 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CXCL16 antibody and an CXCL16-HRP conjugate. The assay sample and buffer are incubated together with CXCL16-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CXCL16 concentration since CXCL16 from samples and CXCL16-HRP conjugate compete for the anti-CXCL16 antibody binding site. Since the number of sites is limited, as more sites are occupied by CXCL16 from the sample, fewer sites are left to bind CXCL16-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CXCL16 concentration in each sample is interpolated from this standard curve.

154816178

NP_001094282.1

NM_001100812.1

27,579 Da

Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Peptide Ligand-binding Receptors Pathway (106358)

CXCL16: Acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis. Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo. Belongs to the intercrine alpha (chemokine CxC) family. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 17p13. Cellular Component: extracellular space; membrane; extracellular region; plasma membrane; integral to membrane. Molecular Function: low-density lipoprotein receptor activity; chemokine activity; scavenger receptor activity. Biological Process: receptor-mediated endocytosis; response to cytokine stimulus; chemotaxis; positive regulation of cell growth; positive regulation of cell migration

48-Strip-Wells

470 USD

96-Strip-Wells

675