ELISA/assay

Mouse CXC-chemokine receptor 1 ELISA Kit

MBS728400

48-Strip-Wells

470 USD

ELISA Kit

Mouse CXC-chemokine receptor 1 ELISA Kit

CXC-chemokine receptor 1

CXCR1; C-X-C chemokine receptor type 1; C-X-C chemokine receptor type 1; CXC-R1; CXCR-1; IL-8R A; IL-8 receptor type 1; interleukin 8 receptor, alpha; interleukin-8 receptor type 1; interleukin-8 receptor type A; high affinity interleukin-8 receptor A; chemokine (C-X-C motif) receptor 1; CDw128a; High affinity interleukin-8 receptor A; IL-8R A; IL-8 receptor type 1; CD_antigen: CD181

CXCR1

CXCR1; CXCR1; C-C; CD128; CD181; CKR-1; IL8R1; IL8RA; CMKAR1; IL8RBA; CDw128a; C-C-CKR-1; CMKAR1; IL8RA; CXC-R1; CXCR-1; IL-8R A

CXCR1_HUMAN

Mouse

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Immunology

Intended Uses: This CXCR1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CXCR1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: CXCR1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CXCR1 antibody and an CXCR1-HRP conjugate. The assay sample and buffer are incubated together with CXCR1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CXCR1 concentration since CXCR1 from samples and CXCR1-HRP conjugate compete for the anti-CXCR1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CXCR1 from the sample, fewer sites are left to bind CXCR1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CXCR1 concentration in each sample is interpolated from this standard curve.

62911779

AAY21515.1

39,791 Da

Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Endocytosis Pathway (102279); Endocytosis Pathway (102181); Epithelial Cell Signaling In Helicobacter Pylori Infection Pathway (83102); Epithelial Cell Signaling In Helicobacter Pylori Infection Pathway (515)

The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. Knockout studies in mice suggested that this protein inhibits embryonic oligodendrocyte precursor migration in developing spinal cord. This gene, IL8RB, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. [provided by RefSeq, Jul 2008]

IL8RA: Receptor to interleukin-8, which is a powerful neutrophils chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activate a phosphatidylinositol-calcium second messenger system. This receptor binds to IL-8 with a high affinity and to MGSA (GRO) with a low affinity. Belongs to the G-protein coupled receptor 1 family. Protein type: Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral; GPCR, family 1; Receptor, cytokine. Chromosomal Location of Human Ortholog: 2q35. Cellular Component: membrane; plasma membrane; integral to membrane. Molecular Function: G-protein coupled receptor activity; interleukin-8 receptor activity; chemokine receptor activity; interleukin-8 binding. Biological Process: G-protein coupled receptor protein signaling pathway; cell surface receptor linked signal transduction; receptor internalization; dendritic cell chemotaxis; chemotaxis; inflammatory response. Disease: Human Immunodeficiency Virus Type 1, Susceptibility To

48-Strip-Wells

470 USD

96-Strip-Wells

675