catalog number :
MBS728391
products type :
ELISA Kit
products full name :
Human Complement Component 4B ELISA Kit
products short name :
Complement Component 4B
other names :
complement component 4B, partial; Complement component 4B; Complement component 4B
uniprot entry name :
R9QA77_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of C4B. No significant cross-reactivity or interference between C4B and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between C4B and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 ng/mL.
other info2 :
Intended Uses: This C4B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human C4B. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Immunology
products description :
Principle of the assay: C4B ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for C4B. Standards or samples are then added to the microtiter plate wells and C4B if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of C4B present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for C4B are added to each well to "sandwich" the C4B immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain C4B and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C4B concentration in each sample is interpolated from this standard curve.
ncbi acc num :
AET24773.1
ncbi mol weight :
1,094 Da
size1 :
48-Strip-Wells-(Competitive)
size2 :
48-Strip-Wells-(Sandwich)
size3 :
96-Strip-Wells-(Competitive)
size4 :
96-Strip-Wells-(Sandwich)