Mouse Hepcidin ELISA Kit | MyBioSource MBS728381 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Hepcidin ELISA Kit

catalog :

MBS728381

quantity :

48-Strip-Wells

price :

440 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS728381

products type :

ELISA Kit

products full name :

Mouse Hepcidin ELISA Kit

products short name :

[Hepcidin]

other names :

[hepcidin preproprotein; Hepcidin; hepcidin; putative liver tumor regressor; liver-expressed antimicrobial peptide 1; hepcidin antimicrobial peptide; Liver-expressed antimicrobial peptide 1; LEAP-1; Putative liver tumor regressor; PLTR]

products gene name :

[Hepc]

other gene names :

[HAMP; HAMP; HEPC; PLTR; HFE2B; LEAP1; HEPC; LEAP1; UNQ487/PRO1003; LEAP-1; PLTR; Hepc25; Hepc20]

uniprot entry name :

HEPC_HUMAN

reactivity :

Mouse

specificity :

This assay has high sensitivity and excellent specificity for detection of HEPC. No significant cross-reactivity or interference between HEPC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HEPC and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Sandwich. Sensitivity: 0.1 ng/mL.

products categories :

Cardiovascular

products description :

Intended Uses: This HEPC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human HEPC. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: HEPC ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for HEPC. Standards or samples are then added to the microtiter plate wells and HEPC if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of HEPC present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for HEPC are added to each well to "sandwich" the HEPC immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain HEPC and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HEPC concentration in each sample is interpolated from this standard curve.

ncbi gi num :

10863973

ncbi acc num :

NP_066998.1

ncbi gb acc num :

NM_021175.2

ncbi mol weight :

9,408 Da

ncbi pathways :

Iron Metabolism In Placenta Pathway (672461)

ncbi summary :

The product encoded by this gene is involved in the maintenance of iron homeostasis, and it is necessary for the regulation of iron storage in macrophages, and for intestinal iron absorption. The preproprotein is post-translationally cleaved into mature peptides of 20, 22 and 25 amino acids, and these active peptides are rich in cysteines, which form intramolecular bonds that stabilize their beta-sheet structures. These peptides exhibit antimicrobial activity. Mutations in this gene cause hemochromatosis type 2B, also known as juvenile hemochromatosis, a disease caused by severe iron overload that results in cardiomyopathy, cirrhosis, and endocrine failure. [provided by RefSeq, Jul 2008]

uniprot summary :

HAMP: Seems to act as a signaling molecule involved in the maintenance of iron homeostasis. Seems to be required in conjunction with HFE to regulate both intestinal iron absorption and iron storage in macrophages. Defects in HAMP are the cause of hemochromatosis type 2B (HFE2B); also known as juvenile hemochromatosis (JH). HFE2B is a disorder of iron metabolism with excess deposition of iron in the tissues, bronze skin pigmentation, hepatic cirrhosis, arthropathy and diabetes. The most common symptoms of hemochromatosis type 2 at presentation are hypogonadism and cardiomyopathy. Belongs to the hepcidin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 19q13.1. Cellular Component: apical cortex; extracellular region. Molecular Function: hormone activity. Biological Process: killing of cells of another organism; cellular iron ion homeostasis; defense response to bacterium; immune response; defense response to fungus. Disease: Hemochromatosis, Type 2b

size1 :

48-Strip-Wells

price1 :

440 USD

size2 :

96-Strip-Wells

price2 :

640

size3 :

5x96-Strip-Wells

price3 :

2895

size4 :

10x96-Strip-Wells

price4 :

5415

more info or order :

MyBioSource product webpage