ELISA/assay

Rat Substance P ELISA Kit

MBS728320

48-Strip-Wells

470 USD

ELISA Kit

Rat Substance P ELISA Kit

Substance P

substance P; Protachykinin-1; protachykinin-1; PPT; tachykinin 1; Tachykinin (substance P, neurokinin A, neuropeptide K, neuropeptide gamma); tachykinin, precursor 1; PPTCleaved into the following 5 chains:Substance P; Neurokinin A; NKAAlternative name(s):Neuromedin L; Substance K

S.P

Tac1; Tac1; TAC; PPTA3; Ppt5fl; RATPPTA3; Nka; Nkna; Tac2; NKA; NPK

TKN1_RAT

Rat

This assay has high sensitivity and excellent specificity for detection of SP. No significant cross-reactivity or interference between SP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SP and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0pg/mL.

Neurobiology

Intended Uses This SP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat SP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: SP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SP. Standards or samples are then added to the microtiter plate wells and SP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for SP are added to each well to "sandwich” the SP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SP concentration in each sample is interpolated from this standard curve.

56068

CAA39752.1

P06767

11,374 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (1010164); G Alpha (q) Signalling Events Pathway (1010197); GPCR Downstream Signaling Pathway (1010193); GPCR Ligand Binding Pathway (1010163); Gastrin-CREB Signalling Pathway Via PKC And MAPK (1010213); Peptide Ligand-binding Receptors Pathway (1010165); Signal Transduction Pathway (1010015); Signaling By GPCR Pathway (1010162); Tachykinin Receptors Bind Tachykinins Pathway (1010167)

encodes neuropeptides substance P, neurokinin A, neuropeptide K, and neuropeptide gamma [RGD, Feb 2006]

48-Strip-Wells

470 USD

96-Strip-Wells

675