ELISA/assay

Human Growth Differentiation Factor 5 ELISA Kit

MBS728296

48-Strip-Wells

470 USD

ELISA Kit

Human Growth Differentiation Factor 5 ELISA Kit

Growth Differentiation Factor 5

GDF5, partial; GDF5; GDF5

GDF5

GDF5

D3YQT0_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of GDF-5. No significant cross-reactivity or interference between GDF-5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GDF-5 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This GDF-5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GDF-5. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cancer

Principle of the assay: GDF-5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GDF-5 antibody and an GDF-5-HRP conjugate. The assay sample and buffer are incubated together with GDF-5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GDF-5 concentration since GDF-5 from samples and GDF-5-HRP conjugate compete for the anti-GDF-5 antibody binding site. Since the number of sites is limited, as more sites are occupied by GDF-5 from the sample, fewer sites are left to bind GDF-5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GDF-5 concentration in each sample is interpolated from this standard curve.

290566063

ADD39410.1

18,733 Da

The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Mutations in this gene are associated with acromesomelic dysplasia, Hunter-Thompson type; brachydactyly, type C; and chondrodysplasia, Grebe type. These associations confirm that the gene product plays a role in skeletal development. [provided by RefSeq, Jul 2008]

GDF5: a cytokine that is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. These cytokines are characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Binds to bone morphogenetic protein receptors (BMPRs), a family of transmembrane serine/threonine kinases. BMPRs are involved in bone and cartilage formation. Chondrogenic signaling is mediated by the high-affinity receptor BMPR1B. Defects in GDF5 are the cause of acromesomelic chondrodysplasia Grebe type (AMDG), acromesomelic chondrodysplasia Hunter-Thompson type (AMDH), brachydactyly type C (BDC), Du Pan syndrome (DPS), symphalangism proximal syndrome (SYM1), multiple synostoses syndrome type 2 (SYNS2), and brachydactyly type A2 (BDA2). Genetic variations in GDF5 are associated with susceptibility to osteoarthritis type 5 (OS5). Belongs to the TGF-beta family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 20q11.2. Cellular Component: extracellular space; plasma membrane; extracellular region. Molecular Function: identical protein binding; protein binding; growth factor activity; cytokine activity; transforming growth factor beta receptor binding. Biological Process: hindlimb morphogenesis; extracellular matrix organization and biogenesis; regulation of multicellular organism growth; BMP signaling pathway; forelimb morphogenesis; regulation of apoptosis; positive regulation of chondrocyte differentiation; cell-cell signaling; transforming growth factor beta receptor signaling pathway; regulation of MAPKKK cascade; chondrocyte differentiation; negative regulation of neuron apoptosis; positive regulation of neuron differentiation; cell development; embryonic limb morphogenesis; negative regulation of epithelial cell proliferation; growth. Disease: Chondrodysplasia, Grebe Type; Acromesomelic Dysplasia, Hunter-thompson Type; Brachydactyly, Type A1, C; Brachydactyly, Type A2; Symphalangism, Proximal, 1b; Brachydactyly, Type C; Fibular Hypoplasia And Complex Brachydactyly; Osteoarthritis Susceptibility 5; Multiple Synostoses Syndrome 2

48-Strip-Wells

470 USD

96-Strip-Wells

675