ELISA/assay

Human Glutathione Peroxidase 1 ELISA Kit

MBS728295

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human Glutathione Peroxidase 1 ELISA Kit

Glutathione Peroxidase 1

glutathione peroxidase 1 isoform 1; Glutathione peroxidase 1; glutathione peroxidase 1; GPx-1; GSHPx-1; cellular glutathione peroxidase; glutathione peroxidase 1; Cellular glutathione peroxidase

GPX1

GPX1; GPX1; GPXD; GSHPX1; GPx-1; GSHPx-1

GPX1_HUMAN

Human

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

Intended Uses: This GP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GP1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: GP1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GP1. Standards or samples are then added to the microtiter plate wells and GP1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GP1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GP1 are added to each well to "sandwich" the GP1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GP1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GP1 concentration in each sample is interpolated from this standard curve.

41406084

NP_000572.2

NM_000581.2

10,289 Da

Amyotrophic Lateral Sclerosis (ALS) Pathway (920975); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (685553); Arachidonic Acid Metabolism Pathway (366); Cellular Responses To Stress Pathway (645258); Detoxification Of Reactive Oxygen Species Pathway (941400); Direct P53 Effectors Pathway (137939); Folate Metabolism Pathway (198833)

This gene encodes a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence. The allele with five ALA repeats is significantly associated with breast cancer risk. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

GPX1: Protects the hemoglobin in erythrocytes from oxidative breakdown. Homotetramer. Interacts with MIEN1. Belongs to the glutathione peroxidase family. Protein type: Other Amino Acids Metabolism - glutathione; EC 1.11.1.9; Lipid Metabolism - arachidonic acid; Oxidoreductase. Chromosomal Location of Human Ortholog: 3p21.3. Cellular Component: mitochondrion; mitochondrial matrix; cytoplasm; nucleus; cytosol. Molecular Function: selenium binding; glutathione binding; phospholipid-hydroperoxide glutathione peroxidase activity; glutathione peroxidase activity; SH3 domain binding. Biological Process: response to nicotine; fat cell differentiation; blood vessel endothelial cell migration; response to toxin; myoblast proliferation; skeletal muscle fiber development; negative regulation of caspase activity; thermoregulation; regulation of gene expression, epigenetic; vasodilation; response to estradiol stimulus; endothelial cell development; regulation of mammary gland epithelial cell proliferation; response to selenium ion; sensory perception of sound; glutathione metabolic process; UV protection; response to symbiotic bacterium; lipoxygenase pathway; hydrogen peroxide catabolic process; response to folic acid; regulation of neuron apoptosis; response to glucose stimulus; arachidonic acid metabolic process; response to gamma radiation; negative regulation of inflammatory response to antigenic stimulus; aging; protein amino acid oxidation; nucleobase, nucleoside and nucleotide metabolic process; skeletal muscle regeneration; angiogenesis involved in wound healing; purine base metabolic process; positive regulation of protein kinase B signaling cascade; response to xenobiotic stimulus; response to reactive oxygen species; triacylglycerol metabolic process; response to hydrogen peroxide; heart contraction; response to lipid hydroperoxide; cell redox homeostasis; purine nucleotide catabolic process; interaction with symbiont; induction of apoptosis by oxidative stress. Disease: Glutathione Peroxidase Deficiency

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675