ELISA/assay

Mouse Defensin Alpha 5 Paneth Cell Specific ELISA Kit

MBS728264

48-Strip-Wells

470 USD

ELISA Kit

Mouse Defensin Alpha 5 Paneth Cell Specific ELISA Kit

Defensin Alpha 5 Paneth Cell Specific

defensin, alpha 5, Paneth cell-specific; Defensin-5; defensin-5; HD5(20-94); defensin 5; defensin, alpha 5, preproprotein; defensin, alpha 5, Paneth cell-specific; Defensin, alpha 5; HD5(20-94)Cleaved into the following 4 chains:HD5(23-94); HD5(29-94); HD5(56-94); HD5(63-94)

DEFalpha5

DEFA5; DEFA5; DEF5; HD-5; DEF5

DEF5_HUMAN

Mouse

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This DEFA5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse DEFA5. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the Assay: DEFA5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DEFA5 antibody and an DEFA5-HRP conjugate. The assay sample and buffer are incubated together with DEFA5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DEFA5 concentration since DEFA5 from samples and DEFA5-HRP conjugate compete for the anti-DEFA5 antibody binding site. Since the number of sites is limited, as more sites are occupied by DEFA5 from the sample, fewer sites are left to bind DEFA5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DEFA5 concentration in each sample is interpolated from this standard curve.

50057850

AAT68886.1

10,071 Da

Alpha-defensins Pathway (530758); Defensins Pathway (530757); Immune System Pathway (106386); Innate Immune System Pathway (106387)

Defensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several of the alpha defensin genes appear to be clustered on chromosome 8. The protein encoded by this gene, defensin, alpha 5, is highly expressed in the secretory granules of Paneth cells of the ileum. [provided by RefSeq, Jul 2008]

DEFA5: Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. All DEFA5 peptides exert antimicrobial activities, but their potency is affected by peptide processing. Belongs to the alpha-defensin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 8p23.1. Cellular Component: extracellular space; transport vesicle; Golgi lumen; extracellular region. Biological Process: killing of cells of another organism; antibacterial humoral response; innate immune response; defense response to fungus

48-Strip-Wells

470 USD

96-Strip-Wells

675