ELISA/assay

Human Vitamin E ELISA Kit

MBS728239

48-Strip-Wells

440 USD

ELISA Kit

Human Vitamin E ELISA Kit

[Vitamin E]

[Vitamin E pathway gene 5 isoform 2; Vitamin E pathway gene 5 isoform 1; Vitamin E pathway gene 5 isoform 2]

[VE]

[TCM_043432]

Human

This assay has high sensitivity and excellent specificity for detection of VDBP. No significant cross-reactivity or interference between VDBP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between VDBP and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 1.0 ug/mL.

Intended Uses: This VDBP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine VDBP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: VDBP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-VDBP antibody and an VDBP-HRP conjugate. The assay sample and buffer are incubated together with VDBP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the VDBP concentration since VDBP from samples and VDBP-HRP conjugate compete for the anti-VDBP antibody binding site. Since the number of sites is limited, as more sites are occupied by VDBP from the sample, fewer sites are left to bind VDBP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VDBP concentration in each sample is interpolated from this standard curve.

590566033

XP_007010115.1

XM_007010053.1

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415