catalog number :
MBS728199
products type :
ELISA Kit
products full name :
Human Free Fatty Acid ELISA Kit
products short name :
Free Fatty Acid
specificity :
This assay has high sensitivity and excellent specificity for detection of FFA. No significant cross-reactivity or interference between FFA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FFA and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.
products description :
Intended Uses: This FFA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human FFA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay FFA ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-FFA antibody and an FFA-HRP conjugate. The assay sample and buffer are incubated together with FFA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FFA concentration since FFA from samples and FFA-HRP conjugate compete for the anti-FFA antibody binding site. Since the number of sites is limited, as more sites are occupied by FFA from the sample, fewer sites are left to bind FFA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FFA concentration in each sample is interpolated from this standard curve.