ELISA/assay

Mouse A Disintegrin And Metalloprotease 9 ELISA Kit

MBS728187

96 Strip Wells

675 USD

ELISA Kit

Mouse A Disintegrin And Metalloprotease 9 ELISA Kit

A Disintegrin And Metalloprotease 9

ADAM9 protein; Disintegrin and metalloproteinase domain-containing protein 9; disintegrin and metalloproteinase domain-containing protein 9; cone rod dystrophy 9; myeloma cell metalloproteinase; cellular disintegrin-related protein; ADAM metallopeptidase domain 9 (meltrin gamma); metalloprotease/disintegrin/cysteine-rich protein 9; ADAM metallopeptidase domain 9; Cellular disintegrin-related protein; Meltrin-gamma; Metalloprotease/disintegrin/cysteine-rich protein 9; Myeloma cell metalloproteinase

ADAM9

ADAM9; ADAM9; MCMP; MDC9; CORD9; Mltng; KIAA0021; MCMP; MDC9; MLTNG; ADAM 9

ADAM9_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of ADAM9. No significant cross-reactivity or interference between ADAM9 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ADAM9 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This ADAM9 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ADAM9. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Sensitivity: The sensitivity in this assay is 0.1ng/mL.

Cell Biology

Principle of the assay: ADAM9 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADAM9 antibody and an ADAM9-HRP conjugate. The assay sample and buffer are incubated together with ADAM9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADAM9 concentration since ADAM9 from samples and ADAM9-HRP conjugate compete for the anti-ADAM9 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADAM9 from the sample, fewer sites are left to bind ADAM9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAM9 concentration in each sample is interpolated from this standard curve.

116496893

AAI26407.1

72,359 Da

Collagen Degradation Pathway 730309!!Degradation Of The Extracellular Matrix Pathway 576263!!Extracellular Matrix Organization Pathway 576262

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene interacts with SH3 domain-containing proteins, binds mitotic arrest deficient 2 beta protein, and is also involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor. Several alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jul 2010]

96 Strip Wells

675 USD