ELISA/assay

Human Melanoma Marker A ELISA Kit

MBS728153

48-Strip-Wells

470 USD

ELISA Kit

Human Melanoma Marker A ELISA Kit

Melanoma Marker A

MART/Melan A

Human

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

Cell Biology

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This MART ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human MART. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY MART ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MART antibody and an MART-HRP conjugate. The assay sample and buffer are incubated together with MART-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MART concentration since MART from samples and MART-HRP conjugate compete for the anti-MART antibody binding site. Since the number of sites is limited, as more sites are occupied by MART from the sample, fewer sites are left to bind MART-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MART concentration in each sample is interpolated from this standard curve.

48-Strip-Wells

470 USD

96-Strip-Wells

675