product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Mouse Citrate Synthase ELISA Kit
catalog :
MBS728120
quantity :
48-Strip-Wells
price :
440 USD
more info or order :
image
image 1 :
product information
catalog number :
MBS728120
products type :
ELISA Kit
products full name :
Mouse Citrate Synthase ELISA Kit
products short name :
[Citrate Synthase]
other names :
[citrate synthase; Citrate synthase; citrate synthase]
products gene name :
[CS]
other gene names :
[gltA; gltA; ECK0709; gluT; icdB; JW0710; gluT; icdB; b0720; JW0710]
uniprot entry name :
CISY_ECOLI
reactivity :
Mouse
specificity :
This assay has high sensitivity and excellent specificity for detection of CS. No significant cross-reactivity or interference between CS and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CS and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 0.1 ug/mL.
products description :
Intended Uses: This CS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CS. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: CS ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CS antibody and an CS-HRP conjugate. The assay sample and buffer are incubated together with CS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CS concentration since CS from samples and CS-HRP conjugate compete for the anti-CS antibody binding site. Since the number of sites is limited, as more sites are occupied by CS from the sample, fewer sites are left to bind CS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CS concentration in each sample is interpolated from this standard curve.
ncbi gi num :
16128695
ncbi acc num :
NP_415248.1
ncbi gb acc num :
NC_000913.3
uniprot acc num :
P0ABH7
ncbi mol weight :
48,015 Da
ncbi pathways :
2-Oxocarboxylic Acid Metabolism Pathway (715161); 2-Oxocarboxylic Acid Metabolism Pathway (717400); Biosynthesis Of Amino Acids Pathway (790558); Biosynthesis Of Amino Acids Pathway (795174); Biosynthesis Of Secondary Metabolites Pathway (148665); Carbon Metabolism Pathway (815459); Carbon Metabolism Pathway (817567); Citrate Cycle (TCA Cycle) Pathway (1008); Citrate Cycle (TCA Cycle) Pathway (288); Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway (405170)
ncbi summary :
The Escherichia coli citrate synthase is a type II enzyme, which appears to be unique to the Gram-negative bacteria. [More information is available at EcoCyc: EG10402].
size1 :
48-Strip-Wells
price1 :
440 USD
size2 :
96-Strip-Wells
price2 :
640
size3 :
5x96-Strip-Wells
price3 :
2895
size4 :
10x96-Strip-Wells
price4 :
5415
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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