Human Cholecystokinin ELISA Kit | MyBioSource MBS728107 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Cholecystokinin ELISA Kit

catalog :

MBS728107

quantity :

48-Strip-Wells-(Comp

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS728107

products type :

ELISA Kit

products full name :

Human Cholecystokinin ELISA Kit

products short name :

Cholecystokinin

other names :

cholecystokinin; Cholecystokinin; cholecystokinin; prepro-cholecystokinin; cholecystokinin triacontatriapeptide; cholecystokinin; (1-49)-CCK58

products gene name :

CCK

other gene names :

CCK; CCK; CCK; CCK58; CCK39; CCK33; CCK25; CCK18; CCK12; CCK8; CCK7; CCK5

uniprot entry name :

CCKN_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of CCK. No significant cross-reactivity or interference between CCK and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CCK and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Sensitivity: 1.0pg/mL.

products categories :

Signal Transduction

products description :

Intended Uses: This CCK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CCK. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay CCK ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CCK. Standards or samples are then added to the microtiter plate wells and CCK if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CCK present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CCK are added to each well to "sandwich" the CCK immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CCK and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCK concentration in each sample is interpolated from this standard curve.

ncbi gi num :

179996

ncbi acc num :

AAA53094.1

ncbi mol weight :

12,669 Da

ncbi pathways :

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); Peptide Ligand-binding Receptors Pathway (106358); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356)

ncbi summary :

Cholecystokinin is a brain/gut peptide. In the gut, it induces the release of pancreatic enzymes and the contraction of the gallbladder. In the brain, its physiologic role is unclear. The cholecystokinin pro-hormone is processed by endo- and exo-proteolytic cleavages. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Mar 2010]

uniprot summary :

CCK: This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion. Belongs to the gastrin/cholecystokinin family. Protein type: Secreted, signal peptide; Hormone; Cell development/differentiation; Secreted. Chromosomal Location of Human Ortholog: 3p22.1. Cellular Component: axon hillock; extracellular space; axon; dendrite; extracellular region; terminal button; perikaryon. Molecular Function: neuropeptide hormone activity; protein binding; hormone activity. Biological Process: caspase activation; positive regulation of protein oligomerization; positive regulation of peptidyl-tyrosine phosphorylation; release of cytochrome c from mitochondria; behavioral fear response; axonogenesis; positive regulation of mitochondrial depolarization; eating behavior; protein kinase C activation; positive regulation of cell proliferation; neuron migration; regulation of sensory perception of pain; signal transduction; negative regulation of appetite

size1 :

48-Strip-Wells-(Competitive)

price1 :

470 USD

size2 :

48-Strip-Wells-(Sandwich)

price2 :

470

size3 :

96-Strip-Wells-(Competitive)

price3 :

675

size4 :

96-Strip-Wells-(Sandwich)

price4 :

675

more info or order :

MyBioSource product webpage