catalog number :
MBS728094
products type :
ELISA Kit
products full name :
Human sCD163/soluble Haemoglobin Scavenger Receptor ELISA Kit
products short name :
sCD163/soluble Haemoglobin Scavenger Receptor
products gene name :
sCD163
specificity :
This assay has high sensitivity and excellent specificity for detection of sCD163. No significant cross-reactivity or interference between sCD163 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between sCD163 and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
products categories :
Immunology
products description :
Intended Uses: This sCD163 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human sCD163. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay SCD163 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-sCD163 antibody and an sCD163-HRP conjugate. The assay sample and buffer are incubated together with sCD163-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sCD163 concentration since sCD163 from samples and sCD163-HRP conjugate compete for the anti-sCD163 antibody binding site. Since the number of sites is limited, as more sites are occupied by sCD163 from the sample, fewer sites are left to bind sCD163-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sCD163 concentration in each sample is interpolated from this standard curve.