ELISA/assay

Human Growth Differentiation Factor 3 ELISA Kit

MBS728091

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human Growth Differentiation Factor 3 ELISA Kit

Growth Differentiation Factor 3

growth/differentiation factor 3; Growth/differentiation factor 3; growth/differentiation factor 3; GDF-3; growth differentiation factor 3

GDF3

GDF3; GDF3; KFS3; MCOP7; MCOPCB6; UNQ222/PRO248; GDF-3

GDF3_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of GDF3. No significant cross-reactivity or interference between GDF3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GDF3 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 pg/mL.

Intended Uses: This GDF3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GDF3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cancer

Principle of the assay: GDF3 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GDF3. Standards or samples are then added to the microtiter plate wells and GDF3 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GDF3 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GDF3 are added to each well to "sandwich" the GDF3 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GDF3 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GDF3 concentration in each sample is interpolated from this standard curve.

10190670

NP_065685.1

NM_020634.1

41,387 Da

The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. [provided by RefSeq, Jul 2008]

GDF3: Defects in GDF3 are the cause of Klippel-Feil syndrome type 3 (KFS3); also called Klippel-Feil syndrome autosomal dominant 3. KFS3 is a skeletal disorder characterized by congenital fusion of cervical vertebrae. It is due to a failure in the normal segmentation of vertebrae during the early weeks of fetal development. The clinical triad consists of short neck, low posterior hairline, and limited neck movement. Defects in GDF3 are the cause of microphthalmia isolated with coloboma type 6 (MCOPCB6); also called isolated colobomatous microphthalmia 6. MCOPCB6 is a disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataract and other abnormalities like cataract may also be present. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure). Defects in GDF3 are a cause of microphthalmia isolated type 7 (MCOP7). MCOP7 is a disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataract and other abnormalities like cataract may also be present. Belongs to the TGF-beta family. Protein type: Secreted; Secreted, signal peptide; Cytokine. Chromosomal Location of Human Ortholog: 12p13.1. Cellular Component: extracellular space; cytoplasm. Molecular Function: growth factor activity; cytokine activity; protein kinase binding; transforming growth factor beta receptor binding. Biological Process: response to dietary excess; in utero embryonic development; notochord development; somite rostral/caudal axis specification; formation of anatomical boundary; negative regulation of BMP signaling pathway; regulation of apoptosis; eye development; regulation of MAPKKK cascade; negative regulation of epidermal cell differentiation; regulation of cell fate commitment; mesoderm development; negative regulation of myoblast differentiation; skeletal development; cell development; growth; endoderm development. Disease: Microphthalmia, Isolated 7; Klippel-feil Syndrome 3, Autosomal Dominant; Microphthalmia, Isolated, With Coloboma 6

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675