catalog number :
MBS728060
products type :
ELISA Kit
products full name :
Rabbit Anti-OVA-IgE ELISA Kit
products short name :
OVA-IgE
products name syn :
Rabbit Anti-OVA-IgE ELISA Kit; Anti-OVA-IgE; Anti-OVA-IgE (Rabbit)
products gene name :
OVA sIgE
specificity :
This assay has high sensitivity and excellent specificity for detection of AntiOVAIgE. No significant cross-reactivity or interference between AntiOVAIgE and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AntiOVAIgE and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
other info2 :
Intended Uses: This AntiOVAIgE ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit AntiOVAIgE. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Rabbit ELISA Kit
products description :
Principle of the assay: AntiOVAIgE ELISA kit applies the competitive enzyme immunoassay technique utilizing ovalbumin antigen and an AntiOVAIgE-HRP conjugate. The assay sample and buffer are incubated together with AntiOVAIgE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AntiOVAIgE concentration since AntiOVAIgE from samples and AntiOVAIgE-HRP conjugate compete for the ovalbumin antigen binding site. Since the number of sites is limited, as more sites are occupied by AntiOVAIgE from the sample, fewer sites are left to bind AntiOVAIgE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AntiOVAIgE concentration in each sample is interpolated from this standard curve.