ELISA/assay

Human Soluble Fms like Tyrosine 1 ELISA Kit

MBS728035

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human Soluble Fms like Tyrosine 1 ELISA Kit

Soluble Fms like Tyrosine 1

soluble fms-like tyrosine kinase 1; Flt1 protein; vascular endothelial growth factor receptor 1; flt1a; receptor tyrosine kinase Flt1b; soluble fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor); Flt1 protein; Soluble Fms-related tyrosine kinase 1/soluble vascular endothelial growth factor receptor 1 isoform sFlt1-11a; Soluble fms-like tyrosine kinase 1

sFLT1

flt1; flt1; flt1b; sFlt1; VEGFR1; sFlt1; sflt1

A4QNT7_DANRE

Human

This assay has high sensitivity and excellent specificity for detection of sFLt-1. No significant cross-reactivity or interference between sFLt-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between sFLt-1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

Intended Uses: This sFLt-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human sFLt-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the assay: sFLt-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-sFLt-1 antibody and an sFLt-1-HRP conjugate. The assay sample and buffer are incubated together with sFLt-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sFLt-1 concentration since sFLt-1 from samples and sFLt-1-HRP conjugate compete for the anti-sFLt-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by sFLt-1 from the sample, fewer sites are left to bind sFLt-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sFLt-1 concentration in each sample is interpolated from this standard curve.

324499394

ADY39752.1

53,549 Da

Adaptive Immune System Pathway (1004110); Constitutive PI3K/AKT Signaling In Cancer Pathway (1003436); Cytokine-cytokine Receptor Interaction Pathway (119260); Cytokine-cytokine Receptor Interaction Pathway (460); DAP12 Interactions Pathway (1004203); DAP12 Signaling Pathway (1004204); Disease Pathway (1003331); Downstream Signal Transduction Pathway (1003616); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (1004121); Downstream Signaling Of Activated FGFR Pathway (1003377)

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675