Mouse Nephrin ELISA Kit | MyBioSource MBS727934 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Nephrin ELISA Kit

catalog :

MBS727934

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS727934

products type :

ELISA Kit

products full name :

Mouse Nephrin ELISA Kit

products short name :

Nephrin

other names :

nephrin; Nephrin; nephrin; renal glomerulus-specific cell adhesion receptor; nephrosis 1, congenital, Finnish type (nephrin); Renal glomerulus-specific cell adhesion receptor

products gene name :

Nephrin

other gene names :

NPHS1; NPHS1; CNF; NPHN; nephrin; NPHN

uniprot entry name :

NPHN_HUMAN

reactivity :

Mouse

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This NPHN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse NPHN. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products description :

Principle of the Assay: NPHN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NPHN antibody and an NPHN-HRP conjugate. The assay sample and buffer are incubated together with NPHN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NPHN concentration since NPHN from samples and NPHN-HRP conjugate compete for the anti-NPHN antibody binding site. Since the number of sites is limited, as more sites are occupied by NPHN from the sample, fewer sites are left to bind NPHN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NPHN concentration in each sample is interpolated from this standard curve.

ncbi gi num :

10441644

ncbi acc num :

AAG17141.1

ncbi mol weight :

130,546 Da

ncbi pathways :

Cell-Cell Communication Pathway (477132); Nephrin Interactions Pathway (187228); Nephrin/Neph1 Signaling In The Kidney Podocyte Pathway (138072)

ncbi summary :

This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.[provided by RefSeq, Oct 2009]

uniprot summary :

NPHS1: Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion. Interacts with CD2AP (via C-terminal domain). Interacts with MAGI1 (via PDZ 2 and 3 domains) forming a tripartite complex with IGSF5/JAM4. Interacts with DDN; the interaction is direct. Self-associates (via the Ig-like domains). Also interacts (via the Ig-like domains) with KIRREL/NEPH1 and KIRREL2; the interaction with KIRREL is dependent on KIRREL glycosylation. Forms a complex with ACTN4, CASK, IQGAP1, MAGI2, SPTAN1 and SPTBN1. Interacts with NPHS2. Specifically expressed in podocytes of kidney glomeruli. Belongs to the immunoglobulin superfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cell adhesion; Membrane protein, integral; Immunoglobulin superfamily. Chromosomal Location of Human Ortholog: 19q13.1. Cellular Component: cell projection; protein complex; integral to plasma membrane; plasma membrane; lipid raft. Molecular Function: protein domain specific binding; protein binding; myosin binding; spectrin binding; alpha-actinin binding. Biological Process: skeletal muscle development; positive regulation of actin filament polymerization; glomerular basement membrane development; JNK cascade; cell adhesion; excretion; myoblast fusion. Disease: Nephrotic Syndrome, Type 1

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage