ELISA/assay

Rat Glyceraldehyde 3 Phosphate Dehydrogenase ELISA Kit

MBS727873

48-Strip-Wells

470 USD

ELISA Kit

Rat Glyceraldehyde 3 Phosphate Dehydrogenase ELISA Kit

Glyceraldehyde 3 Phosphate Dehydrogenase

Glyceraldehyde-3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; peptidyl-cysteine S-nitrosylase GAPDH; 38 kDa BFA-dependent ADP-ribosylation substrate; glyceraldehyde-3-phosphate dehydrogenase; 38 kDa BFA-dependent ADP-ribosylation substrate; BARS-38; Peptidyl-cysteine S-nitrosylase GAPDH (EC:2.6.99.-)

GAPDH

Gapdh; Gapdh; Gapd; BARS-38; Gapd; GAPDH

G3P_RAT

Rat

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.

Neurobiology

Intended Uses: This GAPDH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat GAPDH. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: GAPDH ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GAPDH. Standards or samples are then added to the microtiter plate wells and GAPDH if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GAPDH present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GAPDH are added to each well to "sandwich" the GAPDH immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GAPDH and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GAPDH concentration in each sample is interpolated from this standard curve.

122065190

P04797.3

35,828 Da

Alzheimer's Disease Pathway (83489); Alzheimer's Disease Pathway (509); Androgen Receptor Signaling Pathway (198529); Biosynthesis Of Amino Acids Pathway (791250); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (816129); Carbon Metabolism Pathway (817567); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (434661); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (468196); Glycolysis (Embden-Meyerhof Pathway), Glucose = Pyruvate (434659)

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. A similar protein in human and mouse has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The encoded protein was originally identified as a key glycolytic enzyme that converts D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Subsequent studies in human and mouse have assigned a variety of additional functions to the protein including nitrosylation of nuclear proteins. Many pseudogenes similar to this locus are found throughout the rat genome. [provided by RefSeq, Jan 2014]

GAPDH: a multifunctional enzyme with both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities. A key glycolytic enzyme that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. An important enzyme for energy metabolism, and the production of ATP and pyruvate through anaerobic glycolysis in the cytoplasm. Additionally, it participates in apoptosis, membrane trafficking, iron metabolism, nuclear activities and receptor mediated cell signaling. Its subcellular localization changes reflecting its multiple activities. Is cytosolic, but is also localized in the membrane, the nucleus, polysomes, the ER and the Golgi. Participates in transcription, RNA transport, DNA replication and apoptosis. S-nitrosylation on Cys-152 following apoptotic stimulates its interaction with SIAH2, which in turn moderates its translocation into the nucleus. Mediates cysteine S-nitrosylation of nuclear target proteins including SIRT1, HDAC2 and DNA-PK. Deregulated in lung cancer, renal cancer, breast cancer, gastric cancer, glioma, liver cancer, colorectal cancer, melanoma, prostatic cancer, pancreatic cancer and bladder cancer. Its increased expression and enzymatic activity is associated with cell proliferation and tumorigenesis, Oxidative stress impairs GAPDH catalytic activity and leads to cellular aging and apoptosis. In experimental animal models, injection of GAPDH antagonists induces apoptosis and blocks Hep3B tumor progression, suggesting a therapeutic potential of targeting GAPDH in human hepatocellular carcinoma. Protein type: EC 1.2.1.12; Oxidoreductase; Carbohydrate Metabolism - glycolysis and gluconeogenesis. Cellular Component: microtubule cytoskeleton; nuclear membrane; membrane; intracellular membrane-bound organelle; mitochondrion; cytoplasm; plasma membrane; lipid particle; ribonucleoprotein complex; cytosol; nucleus; vesicle. Molecular Function: identical protein binding; protein binding; microtubule binding; glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity; NADP binding; NAD binding. Biological Process: regulation of translation; neuron apoptosis; glycolysis; protein stabilization; apoptosis; multicellular organismal development; negative regulation of translation; carbohydrate metabolic process; microtubule cytoskeleton organization and biogenesis; gluconeogenesis

48-Strip-Wells

470 USD

96-Strip-Wells

675