ELISA/assay

Mouse Phospho-Glycogen Synthase Kinase 3beta ELISA Kit

MBS727836

48-Strip-Wells

470 USD

ELISA Kit

Mouse Phospho-Glycogen Synthase Kinase 3beta ELISA Kit

Phospho-Glycogen Synthase Kinase 3beta

Mouse Phospho-Glycogen Synthase Kinase 3b ELISA Kit

podocin isoform 1; Podocin; podocin; nephrosis 2, idiopathic, steroid-resistant (podocin)

P-GSK3beta

P-GSK3b

NPHS2; NPHS2; PDCN; SRN1

PODO_HUMAN

Mouse

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This PDCN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse PDCN. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: PDCN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PDCN antibody and an PDCN-HRP conjugate. The assay sample and buffer are incubated together with PDCN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PDCN concentration since PDCN from samples and PDCN-HRP conjugate compete for the anti-PDCN antibody binding site. Since the number of sites is limited, as more sites are occupied by PDCN from the sample, fewer sites are left to bind PDCN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PDCN concentration in each sample is interpolated from this standard curve.

7657615

NP_055440.1

NM_014625.3

34,421 Da

Nephrin/Neph1 Signaling In The Kidney Podocyte Pathway (138072)

This gene encodes a protein that plays a role in the regulation of glomerular permeability. Mutations in this gene cause steroid-resistant nephrotic syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

NPHS2: Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton. Defects in NPHS2 are the cause of nephrotic syndrome type 2 (NPHS2). It is a renal disorder characterized clinically by childhood onset of proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. The disorder is resistant to steroid treatment and progresses to end-stage renal failure in the first or second decades. Some patients show later onset of the disorder. Belongs to the band 7/mec-2 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 1q25.2. Cellular Component: protein complex; endoplasmic reticulum; integral to plasma membrane; plasma membrane; intercellular junction; lipid raft. Molecular Function: protein binding. Biological Process: actin cytoskeleton reorganization; excretion. Disease: Nephrotic Syndrome, Type 2

48-Strip-Wells

470 USD

96-Strip-Wells

675