ELISA/assay

Mouse Matrix metalloproteinsae 1 ELISA Kit

MBS727809

48-Strip-Wells

440 USD

ELISA Kit

Mouse Matrix metalloproteinsae 1 ELISA Kit

[Matrix metalloproteinsae 1]

[matrix metalloproteinase 1 isoform 2; matrix metalloproteinase 1; GLEAN_14266]

[MMP-1]

[Mmp-1; Mmp1]

Mouse

This assay has high sensitivity and excellent specificity for detection of MMP-1. No significant cross-reactivity or interference between MMP-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MMP-1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Detection Range: 1.0-25ng/mL. Sensitivity: 0.1ng/mL

Cancer

Intended Uses: This MMP-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse MMP-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: MMP-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MMP-1 antibody and an MMP-1-HRP conjugate. The assay sample and buffer are incubated together with MMP-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MMP-1 concentration since MMP-1 from samples and MMP-1-HRP conjugate compete for the anti-MMP-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by MMP-1 from the sample, fewer sites are left to bind MMP-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMP-1 concentration in each sample is interpolated from this standard curve.

255958234

NP_001157647.1

NM_001164175.1

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415