ELISA/assay

Human Hyaluronidase ELISA Kit

MBS727688

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human Hyaluronidase ELISA Kit

Hyaluronidase

hyaluronidase; Hyaluronidase-3; hyaluronidase-3; lung carcinoma protein 3; hyaluronoglucosaminidase 3; Hyaluronoglucosaminidase-3; Lung carcinoma protein 3; LuCa-3

HAase

HYAL3; HYAL3; LUCA3; HYAL-3; LUCA-3; LUCA3; Hyal-3; LuCa-3

HYAL3_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of HA. No significant cross-reactivity or interference between HA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HA and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive or Sandwich. Sensitivity: 0.1U/L.

Signal Transduction

Intended Uses This HA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human HA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: HA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for HA. Standards or samples are then added to the microtiter plate wells and HA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of HA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for HA are added to each well to "sandwich” the HA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain HA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HA concentration in each sample is interpolated from this standard curve.

2935328

AAC70915.1

15,865 Da

CS/DS Degradation Pathway (645311); Chondroitin Sulfate Degradation Pathway (413376); Chondroitin Sulfate Degradation Pathway (468247); Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway (645308); Dermatan Sulfate Degradation Pathway (413375); Dermatan Sulfate Degradation Pathway (468240); Disease Pathway (530764); Glycogen Storage Diseases Pathway (980468); Glycosaminoglycan Degradation Pathway (82981); Glycosaminoglycan Degradation Pathway (355)

This gene encodes a member of the hyaluronidase family. Hyaluronidases are endoglycosidase enzymes that degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. The regulated turnover of hyaluronan plays a critical role in many biological processes including cell proliferation, migration and differentiation. The encoded protein may also play an important role in sperm function. This gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression, and the expression of specific transcript variants may be indicative of tumor status. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and some isoforms may lack hyaluronidase activity. This gene overlaps and is on the same strand as N-acetyltransferase 6 (GCN5-related), and some transcripts of each gene share a portion of the first exon. [provided by RefSeq, Jan 2011]

HYAL3: a member of the hyaluronidase family. Hyaluronidases are endoglycosidase enzymes that degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. The regulated turnover of hyaluronan plays a critical role in many biological processes including cell proliferation, migration and differentiation. The encoded protein may also play an important role in sperm function. This gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression, and the expression of specific transcript variants may be indicative of tumor status. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and some isoforms may lack hyaluronidase activity. This gene overlaps and is on the same strand as N-acetyltransferase 6 (GCN5-related), and some transcripts of each gene share a portion of the first exon. [provided by RefSeq, Jan 2011]. Protein type: Hydrolase; Secreted, signal peptide; EC 3.2.1.35; Secreted. Chromosomal Location of Human Ortholog: 3p21.3. Cellular Component: lysosome; extracellular region; cytoplasmic vesicle. Molecular Function: viral receptor activity; hyaluronoglucuronidase activity; hyalurononglucosaminidase activity. Biological Process: response to antibiotic; response to virus; cartilage development; carbohydrate metabolic process; inflammatory response; hyaluronan catabolic process

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675