product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Rabbit Epidermal growth factor ELISA Kit
catalog :
MBS727685
quantity :
48-Strip-Wells
price :
470 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS727685
products type :
ELISA Kit
products full name :
Rabbit Epidermal growth factor ELISA Kit
products short name :
Epidermal growth factor
products name syn :
Rabbit Epidermal growth factor ELISA Kit; Epidermal growth factor; Epidermal growth factor (Rabbit)
other names :
epidermal growth factor
products gene name :
EGF
reactivity :
Rabbit
specificity :
Sensitivity: The sensitivity in this assay is 1.0 pg/mL. Specificity: This assay has high sensitivity and excellent specificity for detection of EGF. No significant cross-reactivity or interference between EGF and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive
products categories :
Rabbit ELISA Kit
products description :
For Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This EGF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit EGF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: EGF ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-EGF antibody and an EGF-HRP conjugate. The assay sample and buffer are incubated together with EGF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EGF concentration since EGF from samples and EGF-HRP conjugate compete for the anti-EGF antibody binding site. Since the number of sites is limited, as more sites are occupied by EGF from the sample, fewer sites are left to bind EGF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EGF concentration in each sample is interpolated from this standard curve.
ncbi gi num :
688072
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
size1 :
48-Strip-Wells
price1 :
470 USD
size2 :
96-Strip-Wells
price2 :
675
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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