Rat Cardiac Troponin I ELISA Kit | MyBioSource MBS727624 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Cardiac Troponin I ELISA Kit

catalog :

MBS727624

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS727624

products type :

ELISA Kit

products full name :

Rat Cardiac Troponin I ELISA Kit

products short name :

Cardiac Troponin I

other names :

TNNI3; TNNI3 protein; troponin I, cardiac muscle; cardiomyopathy, dilated 2A (autosomal recessive); troponin I type 3 (cardiac); TNNI3 protein; Troponin I type 3 (Cardiac); Troponin I, cardiac; Troponin I, cardiac variant

products gene name :

cTnI

other gene names :

TNNI3; TNNI3; CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF

uniprot entry name :

Q6FGX2_HUMAN

reactivity :

Rat

specificity :

This assay has high sensitivity and excellent specificity for detection of cTnI. No significant cross-reactivity or interference between cTnI and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between cTnI and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Quantitative Competitive. Sensitivity: 0.1 ng/mL.

products categories :

Cardiovascular

products description :

Intended Uses: This cTnI ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat cTnI. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: cTnI ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-cTnI antibody and an cTnI-HRP conjugate. The assay sample and buffer are incubated together with cTnI-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the cTnI concentration since cTnI from samples and cTnI-HRP conjugate compete for the anti-cTnI antibody binding site. Since the number of sites is limited, as more sites are occupied by cTnI from the sample, fewer sites are left to bind cTnI-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The cTnI concentration in each sample is interpolated from this standard curve.

ncbi gi num :

49456923

ncbi acc num :

CAG46782.1

uniprot acc num :

Q6FGX2

ncbi mol weight :

24,008 Da

ncbi pathways :

Adrenergic Signaling In Cardiomyocytes Pathway (908257); Adrenergic Signaling In Cardiomyocytes Pathway (909696); Cardiac Progenitor Differentiation Pathway (712094); Cardiac Muscle Contraction Pathway (93344); Cardiac Muscle Contraction Pathway (93992); Dilated Cardiomyopathy Pathway (121494); Dilated Cardiomyopathy Pathway (121285); Hypertrophic Cardiomyopathy (HCM) Pathway (114229); Hypertrophic Cardiomyopathy (HCM) Pathway (106591); Muscle Contraction Pathway (106261)

ncbi summary :

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]

uniprot summary :

TNNI3: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1. Belongs to the troponin I family. Protein type: Actin-binding; Motility/polarity/chemotaxis; Motor. Chromosomal Location of Human Ortholog: 19q13.4. Cellular Component: sarcomere; troponin complex; cytosol. Molecular Function: troponin T binding; protein domain specific binding; troponin C binding; protein binding; metal ion binding; calcium channel inhibitor activity; actin binding; calcium-dependent protein binding; protein kinase binding. Biological Process: regulation of smooth muscle contraction; cellular calcium ion homeostasis; heart contraction; heart development; regulation of systemic arterial blood pressure by ischemic conditions; ventricular cardiac muscle morphogenesis; vasculogenesis; negative regulation of ATPase activity; cardiac muscle contraction; muscle filament sliding. Disease: Cardiomyopathy, Dilated, 1ff; Cardiomyopathy, Familial Restrictive, 1; Cardiomyopathy, Familial Hypertrophic, 7; Cardiomyopathy, Dilated, 2a

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage