ELISA/assay

Rat cross linked N-telopeptide of type I collagen, NTX ELISA Kit

MBS727573

48-Strip-Wells

470 USD

ELISA Kit

Rat cross linked N-telopeptide of type I collagen, NTX ELISA Kit

cross linked N-telopeptide of type I collagen, NTX

Rat cross linked N-telopeptide of type I collagen, NTX ELISA Kit; cross linked N-telopeptide of type I collagen, NTX; cross linked N-telopeptide of type I collagen, NTX ELISA Kit (Rat)

NTX

NTX

Rat

This assay has high sensitivity and excellent specificity for detection of NTX. No significant cross-reactivity or interference between NTX and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NTX and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 nmol/L.

Rat ELISA Kit

Intended Uses This NTX ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat NTX. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: NTX ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NTX antibody and an NTX-HRP conjugate. The assay sample and buffer are incubated together with NTX-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NTX concentration since NTX from samples and NTX-HRP conjugate compete for the anti-NTX antibody binding site. Since the number of sites is limited, as more sites are occupied by NTX from the sample, fewer sites are left to bind NTX-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NTX concentration in each sample is interpolated from this standard curve.

4579738

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48-Strip-Wells

470 USD

96-Strip-Wells

675