Rat Glutathione peroxidase ELISA Kit | MyBioSource MBS727547 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Glutathione peroxidase ELISA Kit

catalog :

MBS727547

quantity :

48-Strip-Wells

price :

440 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS727547

products type :

ELISA Kit

products full name :

Rat Glutathione peroxidase ELISA Kit

products short name :

[Glutathione peroxidase]

other names :

[glutathione peroxidase; Glutathione peroxidase 3; glutathione peroxidase 3; GPx-3; plasma glutathione peroxidase; extracellular glutathione peroxidase; glutathione peroxidase 3 (plasma); Extracellular glutathione peroxidase; Plasma glutathione peroxidase; GPx-P; GSHPx-P]

products gene name :

[GSH-Px]

other gene names :

[GPX3; GPX3; GPx-P; GSHPx-3; GSHPx-P; GPXP; GPx-3; GSHPx-3; GPx-P; GSHPx-P]

uniprot entry name :

GPX3_HUMAN

reactivity :

Rat

specificity :

This assay has high sensitivity and excellent specificity for detection of GSH-PX. No significant cross-reactivity or interference between GSH-PX and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GSH-PX and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

image1 heading :

Typical Testing Data/Standard Curve

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Detection Range: 5.0-100ng/mL. Sensitivity: 1.0ng/ml

products description :

Intended Uses: This GSH-PX ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat GSH-PX. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay GSH-PX ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GSH-PX antibody and an GSH-PX-HRP conjugate. The assay sample and buffer are incubated together with GSH-PX-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GSH-PX concentration since GSH-PX from samples and GSH-PX-HRP conjugate compete for the anti-GSH-PX antibody binding site. Since the number of sites is limited, as more sites are occupied by GSH-PX from the sample, fewer sites are left to bind GSH-PX-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GSH-PX concentration in each sample is interpolated from this standard curve.

ncbi gi num :

312004115

ncbi acc num :

CAA41228.2

uniprot acc num :

P22352

ncbi mol weight :

25,552 Da

ncbi pathways :

Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (366); Cellular Responses To Stress Pathway (645258); Detoxification Of Reactive Oxygen Species Pathway (941400); Folate Metabolism Pathway (198833); Glutathione Metabolism Pathway (82973); Glutathione Metabolism Pathway (198824); Glutathione Metabolism Pathway (343); Oxidative Stress Pathway (198916); Selenium Metabolism And Selenoproteins Pathway (198846)

ncbi summary :

This gene product belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. It contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jul 2008]

uniprot summary :

GPX3: Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. Belongs to the glutathione peroxidase family. Protein type: Lipid Metabolism - arachidonic acid; Oxidoreductase; EC 1.11.1.9; Other Amino Acids Metabolism - glutathione; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 5q33.1. Cellular Component: extracellular space; extracellular region. Molecular Function: selenium binding; glutathione peroxidase activity; transcription factor binding. Biological Process: response to reactive oxygen species; hydrogen peroxide catabolic process; response to lipid hydroperoxide; protein homotetramerization

size1 :

48-Strip-Wells

price1 :

440 USD

size2 :

96-Strip-Wells

price2 :

640

size3 :

5x96-Strip-Wells

price3 :

2895

size4 :

10x96-Strip-Wells

price4 :

5415

more info or order :

MyBioSource product webpage